美洲大蠊糖蛋白对2种益生菌生长及其代谢产物的影响

Effects of Glycoprotein from Periplaneta americana on the Growthand Metabolites of Two Probiotic Bacteria

目的:本实验旨在探究美洲大蠊糖蛋白(PAG)对植物乳杆菌和青春双歧杆菌生长及其代谢产物的影响。方法:在含不同浓度PAG的培养基(MRS培养基中PAG浓度分别为0.5、1.0、2.0、4.0 mg/mL;TPY培养基PAG浓度分别为1.0、2.0、4.0、8.0 mg/mL)中培养有益菌,并以菊粉为阳性对照,测定菌液的OD600值、pH以及代谢产物乳酸、短链脂肪酸(SCFAs,即乙酸、丙酸和丁酸)的含量。结果:PAG添加量达1.0 mg/mL时,促植物乳杆菌生长达到最佳,而青春双歧杆菌生长达到最佳时PAG浓度为2.0 mg/mL。添加PAG;1.0 mg/mL)的植物乳杆菌菌液的OD600值、乳酸和SCFAs产量较同浓度菊粉升高,而pH显著(P<0.05)降低。添加PAG(2.0 mg/mL)的青春双歧杆菌菌液的OD600值、丙酸和丁酸产量较同浓度菊粉升高,乳酸产量12 h优于菊粉,乙酸产量24 h优于菊粉,而pH降低,提示PAG促青春双歧杆菌产乳酸和SCFAs的效果与菊粉不相同。结论:PAG能够促进植物乳杆菌和青春双歧杆菌的增殖和有机酸量的生成,效果优于菊粉。

Objective:The study aimed to investigate the effects of Periplaneta americana glycoprotein(PAG)on the growth and metabolites of Lactobacillus plantarum and Bifidobacterium adolescentis.Methods:With inulin as a positive control,the probiotic bacteria were cultured in medium containing different concentrations of PAG(MRS medium with PAG concentrations of 0.5,1.0,2.0,and 4.0 mg/mL,respectively.TPY medium with PAG concentrations of 1.0,2.0,4.0,and 8.0 mg/mL,respectively)to determine the OD600 and pH,and the contents of lactic acid,short-chain fatty acid(SCFA,snamely acetic acid,propionic acid and butyric acid)in the bacterial solution.Results:The growth of Lactobacillus plantarum was greatest when the concentration of PAG reached 1.0 mg/mL,while Bifidobacterium adolescentis grew best with a PAG concentration of 2.0 mg/mL.The OD600 value,production of lactic acid and SCFAs of Lactobacillus plantarum solution at 1.0 mg/mL PAG were higher than those of inulin,while the pH value was significantly lower.In the bacterial solution of Bifidobacterium adolescentis with 2.0 mg/mL PAG,the values of OD600 and the production of propionic acid and butyric acid increased compared to the same concentration of inulin,the lactic acid production was better than that of inulin at 12 h,the acetic acid production was better than that of inulin at 24 h,and the pH value decreased,indicating that PAG and inulin had different effects on promoting Bifidobacterium adolescentis to produce lactic acid and SCFAs.Conclusion:The PAG could promote the proliferation and organic acid production of Lactobacillus plantarum and Bifidobacterium adolescentis,and the effect was better than that of inulin.