脂代谢相关基因ABHD5抑制肾癌细胞体外迁移及侵袭

Lipid metabolism-related gene ABHD5 inhibits renal cancer cell migration and invasion in vitro

目的:探讨透明细胞肾细胞癌(clear cell renal cell cancinoma,ccRcc)细胞体外迁移及侵袭过程中与α/β自水解酶结构域5(α/β-hydrolase domain-containing 5,ABHD5)的关系。方法:采用TCGA数据库分析人ccRcc组织及正常肾组织中ABHD5的表达情况。采用ABHD5过表达慢病毒转染人肾透明细胞癌细胞株786-O及Caki-1后,运用real-time PCR及Western blot技术检测ABHD5表达改变情况。采用Transwell实验、划痕实验检测此基因对细胞体外迁移及侵袭能力的影响。结果:TCGA数据库显示ccRcc组织中ABHD5表达较正常肾脏组织显著下降(P=0.000),且其下降程度在复发转移肿瘤中进一步增加(P=0.007),与患者生存呈显著负相关(P<0.001)。Transwell实验、划痕实验显示过表达ABHD5显著抑制肾癌细胞体外迁移及侵袭能力(P<0.05)。过表达ABHD5通过影响Sting信号进而影响肾癌细胞体外迁移及侵袭。结论:脂代谢相关基因ABHD5抑制ccRcc癌细胞体外迁移及侵袭能力。

Objective:To investigated the relationship between clear cell renal cell carcinoma(ccRcc)cells andα/β-hydrolase domain-containing 5(ABHD5)during migration and invasion in vitro.Methods:The TCGA database was used to analyze the expres-sion of ABHD5 in human ccRcc tissues and normal kidney tissues.After transfection of human ccRcc cell lines 786-O and Caki-1 with ABHD5-overexpressing lentivirus,the changes in ABHD5 expression were detected using real-time PCR and Western blot.Tran-swell assay and scratch assay were used to examine the effects of ABHD5 on cell migration and invasion in vitro.Results:TCGA data-base showed that the expression of ABHD5 in ccRcc tissues was significantly decreased compared with normal renal tissues(P=0.000),and the decrease was greater in recurrent metastatic tumors(P=0.007),which was significantly negatively correlated with the survival of the patients(P<0.001).Transwell assay and scratch assay showed that ABHD5 overexpression significantly inhibited the migration and invasion of ccRcc cells in vitro(P<0.05).Overexpression of ABHD5 inhibited renal cancer cell migration and invasion in vitro by influencing the Sting signaling pathway.Conclusion:Lipid metabolism-related gene ABHD5 inhibited the migration and in-vasion of ccRcc cells in vitro.