摘要
目的检测分析非小细胞肺癌(NSCLC)病人血清微小RNA(miR)-455和miR-383的表达水平,并探讨其临床诊断价值。方法选取我院2020年3月~2023年1月收治的98例NSCLC病人为NSCLC组,同期收治的98例肺部良性疾病病人为肺良性病变组,同期98例体检健康志愿者为健康组。采用实时荧光定量PCR(RT-qPCR)法检测血清miR-455和miR-383的表达水平。Pearson分析NSCLC病人血清miR-455表达水平与miR-383水平的相关性;多元Logistic回归分析及ROC曲线分析NSCLC发生的影响因素及诊断价值。结果健康组、肺良性病变组、NSCLC组血清miR-455、miR-383表达水平依次降低。Pearson分析显示,NSCLC病人血清miR-455表达水平与miR-383水平呈正相关(r=0.582,P<0.05)。Logistic分析发现,miR-455、miR-383低表达是影响NSCLC发生的危险因素(P<0.05)。ROC曲线分析显示,miR-455和miR-383联合诊断NSCLC的AUC显著大于miR-455单独诊断的AUC(Z=3.604,P=0.000)及miR-383单独诊断的AUC(Z=2.594,P=0.010)。结论NSCLC病人血清miR-455、miR-383相对表达水平明显下调,二者联合检测对NSCLC具有较高的诊断价值。
Objective To detect and analyze the expression levels of serum microRNA(miR)-455 and miR-383 in patients with non-small cell lung cancer(NSCLC),and to explore their clinical diagnostic value.Methods A total of 98 NSCLC patients in our hospital from March 2020 to January 2023 were regarded as the NSCLC group,98 patients with benign lung diseases admitted during the same period were regarded as the benign lung disease group,and another 98 healthy volunteers who underwent physical examination in our hospital during the same period were collected as the health group.Real-time fluorescence quantitative PCR(RT-qPCR)method was applied to detect the expression levels of miR-455 and miR-383 in serum.Pearson was applied to analyze the correlation between serum miR-455 expression level and miR-383 level in NSCLC patients;multivariate Logistic regression and ROC curve was applied to analyze the influencing factors and diagnostic value of NSCLC occurrence.Results The expression levels of miR-455 and miR-383 in the serum of the healthy group,benign lung disease group,and NSCLC group decreased sequentially.Pearson analysis showed there was a positive correlation between serum miR-455 expression level and miR-383 level in NSCLC patients(r=0.582,P<0.05).Logistic analysis found that low expression levels of miR-455 and miR-383 were risk factors for the occurrence of NSCLC(P<0.05).ROC curve analysis showed that the AUC of NSCLC diagnosed by the combination of miR-455 and miR-383 was obviously higher than that diagnosed by miR-455 alone(Z=3.604,P=0.000)and miR-383 alone(Z=2.594,P=0.010).Conclusion The relative expression levels of miR-455 and miR-383 in serum of NSCLC patients are obviously down-regulated.The combined detection of the two has high diagnostic value for NSCLC.
作者
高超
戴希勇
蒋钰辉
刘小玉
李晶
熊浩
GAO Chao;DAI Xiyong;JIANG Yuhui;LIU Xiaoyu;LI Jing;XIONG Hao(Department of Surgery,Wuhan Pulmonary Hospital,Wuhan 430030,China;不详)
出处
《临床外科杂志》
2024年第6期607-610,共4页
Journal of Clinical Surgery
基金
湖北省自然科学基金项目(WJ2019F091)。