摘要
目的:用蛋白组学和质谱技术探索细菌性阴道病(BV)患者阴道微环境中的关键蛋白,为探讨BV的发病机制提供新的思路。方法:收集2019年1月至2019年10月在北京大学第一医院门诊和体检中心就诊的10例BV女性和10例健康女性的阴道分泌物,采用蛋白分析技术筛选差异蛋白,通过GO(Gene Ontology)富集、KEGG(Gene Ontology Enrichment Analysis)富集和蛋白质-蛋白质相互作用(PPI)筛选与BV相关的关键蛋白。结果:鉴定到人源化的蛋白2136个,其中差异蛋白142个,上调蛋白104个,下调蛋白38个,涉及的生物学过程主要包括蛋白折叠、蛋白去甲酰化、细胞质翻译和蛋白结合等。鉴定到细菌源性蛋白760个,其中差异蛋白56个,上调蛋白32个,下调蛋白24个,涉及的生物学过程主要包括葡萄糖代谢、糖酵解、膜转运和ATP水解。BV患者阴道分泌物中的烯醇化酶含量明显高于健康女性,差异有统计学意义(P<0.05)。结论:蛋白组学和质谱技术能筛选出可能参与BV发病机制的相关蛋白和信号通路,如烯醇化酶,为阐明其发生发展的过程提供重要信息,有可能为临床诊断和治疗提供重要的靶标。
Objective:To explore key proteins in the vaginal microenvironment of bacterial vaginosis(BV)using proteomics and mass spectrometry techniques.Method:The vaginal secretions of 10 BV women and 10 healthy women who visited the outpatient and physical examination centers of Peking University First Hospital from January 2019 to October 2019 were collected.Differential protein analysis techniques were used to screen for differential proteins,and key proteins related to BV were screened for GO(Gene Ontology)enrichment,KEGG(Gene Ontology Enrichment Analysis)enrichment,and protein protein interaction(PPI)enrichment.Result:2136 humanized proteins were identified,including 142 differentially expressed proteins,104 upregulated proteins,and 38 downregulated proteins.The biological processes involved mainly include protein folding,protein demethylation,cytoplasmic translation,and protein binding.760 bacterial derived proteins were identified,including 56 differential proteins,32 upregulated and 24 downregulated.The biological processes involved mainly include glucose metabolism,glycolysis,membrane transport,and ATP hydrolysis.The content of enolase in vaginal secretions of BV patients was significantly higher than that of healthy women,and the difference was statistically significant(P<0.05).Conclusion:Proteomics and mass spectrometry techniques can screen for relevant proteins and signaling pathways that may be involved in the pathogenesis of BV,such as enolase,providing important information for elucidating its occurrence and development process,and potentially providing important targets for clinical diagnosis and treatment.
作者
张瑞
李昶
马爱昕
刘朝晖
Zhang Rui;Li Chang;Ma Aixin(Department of Obstetrics and Gynecology,Peking University First Hospital,Beijing 100034)
出处
《现代妇产科进展》
2024年第7期488-492,500,共6页
Progress in Obstetrics and Gynecology
基金
国家自然科学基金资助项目(No:81471420)
教育部博士点新教师基金项目(No:20130001120051)。