食管胃交界腺癌和远端胃腺癌中HER2、RKIP及ERK表达的对比研究

Comparative Study of HER2,RKIP and ERK Expression in Adenocarcinoma of Esophagogastric Junction and Distal Gastric Adenocarcinoma

目的:比较HER2、RKIP和ERK在食管胃交界腺癌(adenocarcinoma of esophagogastric junction,AEG)及远端胃腺癌(distal gastric adenocarcinoma,DGA)中的表达情况,并探讨AEG与DGA发病机制的差异。方法:通过免疫组化法检测AEG、DGA及正常胃黏膜组织中HER2、RKIP及ERK的表达情况,分析HER2、RKIP及ERK与临床病理学特征的关系及三者相关性。结果:AEG中HER2阳性表达率高于DGA,差异有统计学意义(P<0.05)。AEG及DGA中RKIP阳性表达率均低于正常胃黏膜组织,ERK阳性表达率均高于正常胃黏膜组织(P<0.05)。AEG中,RKIP在高/中分化、淋巴结转移组中阳性表达率较低,ERK在低分化、浸润浆膜层、淋巴结转移组中阳性表达率较高(P<0.05);HER2与ERK表达正相关(r=0.352,P=0.007),RKIP与ERK表达负相关(r=-0.521,P=0.000)。DGA中,RKIP在低分化、淋巴结转移组中阳性表达率较低,ERK在淋巴结转移组中阳性表达率较高(P<0.05);RKIP与ERK表达负相关(r=-0.287,P=0.021)。结论:AEG中HER2阳性表达率高于DGA,ERK高表达与AEG患者临床病理特征的关系更为密切,RKIP表达与AEG和DGA的分化程度和淋巴结转移情况密切相关。HER2、RKIP、ERK在AEG与DGA中的表达不同,提示不同部位胃腺癌的MAPK信号通路调节机制并不完全一致。

Objective:To compare the expression of HER2,RKIP,and ERK in adenocarcinoma of esophagogastric junction(AEG)and distal gastric adenocarcinoma(DGA),and explore the differences in the pathogenesis of AEG and DGA.Methods:The expression of HER2,RKIP and ERK in AEG,DGA and normal gastric mucosal tissue were detected by immunohistochemistry,and the relationship between HER2,RKIP,and ERK with clinical pathological characteristics and their correlation were analyzed.Results:The positive expression rate of HER2 in AEG was higher than that in DGA,and the difference was statistically significant(P<0.05).The positive expression rates of RKIP in AEG and DGA were lower than that in normal gastric mucosal tissue,while the positive expression rates of ERK were higher than that in normal gastric mucosal tissue(P<0.05).In AEG,the positive expression rate of RKIP was lower in the high/moderate differentiation group and lymph node metastasis group,while the positive expression rate of ERK was higher in the low differentiation group,serosa invasion group,and lymph node metastasis group(P<0.05);HER2 expression was positively correlated with ERK expression(r=0.352,P=0.007),while RKIP expression was negatively correlated with ERK expression(r=-0.521,P=0.000).In DGA,the positive expression rate of RKIP was lower in the poorly differentiated group and lymph node metastatic group,while the positive expression rate of ERK was higher in the lymph node metastatic group(P<0.05);RKIP expression was negatively correlated with ERK expression(r=-0.287,P=0.021).Conclusion:The positive expression rate of HER2 in AEG is higher than that in DGA,and the high expression of ERK is more closely related to the clinicopathological characteristics of AEG patients.The expression of RKIP is closely related to the differentiation degree and lymph node metastasis of AEG and DGA.The expression of HER2,RKIP,and ERK in AEG and DGA is different,indicating that the regulatory mechanism of MAPK signaling pathway is not completely consistent in different locations of gastric adenocarcinoma.