柽柳属植物盐腺的扫描电镜制样方法比较及观察

Comparison of sample preparation methods and observation on salt glands of Tamarix using scanning electron microscope

为研究柽柳属(Tamarix)植物的盐腺特征,本研究首先采用CO_(2)临界点干燥法和冷冻扫描电镜(Cryo-SEM)制样法两种方法,分别对刚毛柽柳(T.hispida)组培苗和土培苗的叶片进行前处理,并在扫描电镜下观察比较盐腺的形态特征差异。结果发现,对于幼嫩的组培苗,Cryo-SEM制样镜检效果好于CO_(2)临界点干燥制样,盐腺更少变形;对于肉质化的土培苗,CO_(2)临界点干燥制样效果更好,分辨率和衬度较好。接着本研究对10种柽柳土培苗叶片进行了常规干燥制样和扫描电镜观察,并统计了盐腺数量和面积,结果可见长穗柽柳(T.elongata)的盐腺数量和面积均为最高。本研究比较了两种制样法对观察柽柳盐腺形态的优劣且进一步研究了10种柽柳叶片的盐腺,为柽柳抗盐相关基础和应用研究提供一定的参考。

In this study,the leaves of tissue and soil culture seedlings of Tamarix hispida were pretreated by CO_(2) critical point drying method and Cryo-scanning electron microscope(SEM)sample preparation method.The morphological characteristics of salt glands were observed and compared by SEM.The result showed that the effect of Cryo-SEM method was better than that of CO_(2) critical point drying method for young tissue culture seedlings.For fleshy soil culture seedlings,the CO_(2) critical point drying method was better than the Cryo-SEM method.Subsequently,this study conducted the CO_(2) critical point drying method and SEM observation on the leaves of soil culture seedlings of 10 Tamarix species,and counted the number and area of salt glands.The result showed that T.elongata had the highest number and area of salt glands.The advantage and disadvantage of the two sample preparation methods were presented based on the salt gland morphology of Tamarix,and the salt glands of 10 Tamarix leaves were further studied.This work provides a reference for the basic and applied research on salt resistance of Tamarix.