富白细胞-血小板纤维蛋白在大鼠牙髓修复中的应用

Application of leukocyte⁃and platelet⁃rich fibrin in dental pulp repair of rat

目的探讨富白细胞-血小板纤维蛋白(leukocyte-and platelet-rich fibrin,L-PRF)作为盖髓剂对大鼠牙髓炎症调节的作用。方法脂多糖(LPS)构建牙髓炎大鼠模型,30只大鼠随机分成5组,即正常对照组、LPS造模组、iRoot BP PLUS组、L-PRF组、L-PRF+iRoot BP PLUS组。直接盖髓14 d后取标本行显微CT(Micro-CT)扫描、苏木精-伊红(HE)及Masson染色、定量实时聚合酶链式反应(qRT-PCR)检测,观察牙髓炎症及硬组织形成程度。结果Micro-CT示:LPS组未见硬组织形成,L-PRF组冠髓内可见斑块状钙化物,BP组及联合组在穿髓孔处见较完整的钙化桥。组织学染色示:LPS组炎症最重、无硬组织形成;联合组炎症最轻,硬组织形成最完整、连续,两组差异有统计学意义(P<0.01);BP组与联合组的炎症及硬组织形成程度均差异无统计学意义(P>0.05)。qRT-PCR结果示:各实验组牙髓组织中TNF-α、IL-1β、IL-6 mRNA相对表达水平相较于LPS组均有所降低,但L-PRF组与LPS组的差异无统计学意义(P>0.05)。结论L-PRF联合iRoot BP PLUS作为复合盖髓材料,可用于大鼠牙髓炎牙髓组织修复以及牙髓炎症调节。

Objective To explore the effect of leukocyte-and platelet-rich fibrin(L-PRF)as a capping agent in the modulation of inflammation in rat dental pulp.Methods The rat model of pulpitis was established by lipopolysaccharide(LPS).A total of 30 Sprague-Dawley rats were randomly divided into five groups:Normal control group,Model group,iRoot BP PLUS group,L-PRF group,iRoot BP PLUS+L-PRF group.Samples were collected on the 14th day after pulp capping.Micro-CT scans,HE and Masson staining,qRT-PCR assays were performed to observe pulp tissue inflammatory and hard tissue formation.Results Micro-CT results:no hard tissue formation was seen in the LPS group,plaque calculus was seen in the L-PRF group,and a more complete calcified bridge was seen at the perforation of the pulp in the BP and combined groups;HE and Masson staining results:the LPS group had the most severe inflammation and no hard tissue formation;the combined group had the least inflammation and the most complete and continuous hard tissue formation,and there was a statistical difference between the two groups(P<0.01);There was no statistical difference in the degree of inflammatory reaction and hard tissue formation between the BP group and the combined group(P>0.05);qRT-PCR results:the relative expression levels of TNF-α,IL-1β,and IL-6 mRNA in the pulp tissues of all experimental groups were reduced compared with those of the LPS group,but the differences between the L-PRF and LPS groups were not statistically significant(P>0.05).Conclusion The combination of L-PRF and iRoot BP PLUS as a composite pulp capping material can be used for the repair of dental pulp tissue and the regulation of inflammation in dental pulpitis in rats.