西瓜深绿色果皮底色基因初步定位及候选基因预测

Preliminary mapping of dark green fruit rind color in watermelon and candidate genes selection

果皮颜色是西瓜重要的外观性状,国内外学者研究发现,在不同遗传背景下,其调控基因不同.以深绿和浅绿果皮底色小果型西瓜品系K2,L1为母本和父本构建F_(2)遗传群体,对所构建遗传群体开展了果皮底色的基因初步定位.在实验室前期将高密度遗传图谱定位果皮底色基因FRC在八号染色体2.01 Mb的基础上,将FRC基因定位到了83.5 kb区段内(27 911 305~27 994 761bp).根据西瓜基因组“97103V2”的注释信息,发现在目标区段内包含15个已注释的基因,初步推测Cla97C08G161570为西瓜果皮底色候选基因,编码2-植基-1,4-萘醌甲基转移酶,该酶参与叶绿醌的生物合成,研究结果为西瓜果皮底色育种和精细定位果皮底色基因打下了基础.

Fruit rind color is an important appearance trait of watermelon,domestic and foreign scholars have found that the regulatory genes are different in different genetic backgrounds.Preliminary gene mapping of pericarp background color was carried out with the F2 genetic population developed by using the dark green and light green small-sized watermelon lines K2 and L1 as maternal and paternal parents.In the early laboratory phase,used a high-density genetic map to locate the FRC gene within a 2.01 Mb region.The FRC gene was located in 83.5 kb region(27911305~27994761 bp).There are 15 annotated genes in the candidate region according to the annotation information of the watermelon genome“97103V2”,respectively,Cla97C08G161570 is a candidate gene for watermelon rind background color.ClCG08G017810 encodes 2-phytyl-1,4-beta-naphthoquinone methyltransferase,chloroplastic,an enzyme involved in the biosynthesis of chloroquinone.The results laid a foundation for the breeding of pericarp background color and the fine localization of pericarp background color genes.