不同栽培型甜菜类黄酮代谢物的比较代谢组学分析

Comparative Metabolomics Analysis of Flavonoid Metabolites in Different Cultivated Beets

【目的】本研究旨在对糖甜菜和红甜菜根中类黄酮成分及差异进行分析。【方法】利用比较代谢组学对两种甜菜根中的类黄酮物质进行比对。【结果】两种甜菜根共检测出6类69种类黄酮物质。差异物质30种,根据代谢物Log2FC判定差异倍数较大的成分有6种,分别为多甲基黄酮类衍生物、异鼠李素、鼠李素糖苷类、万寿菊素糖苷类、香叶木苷和金圣草黄素。在红甜菜块根中有5种差异代谢物含量高于糖甜菜块根,25种差异代谢物含量低于糖甜菜块根。与糖甜菜块根相比,红甜菜块根中黄酮类代谢产物相对含量高的成分是牡荆素、异牡荆素糖苷和芹菜素糖苷。在黄酮和黄酮醇的生物合成途径上,有7种物质被注释到KEGG通路上。【结论】两种甜菜材料中的类黄酮成分具有明显差异,可作为特征物质进一步分析。

【Objective】The purpose of this study was to analyze the flavonoid components and their differences in sugar beet and red beet root.【Methods】The flavonoids in two kinds of beet root were compared by comparative metabolomics.【Results】A total of 69 flavonoids of 6 kinds were detected in two kinds of beetroot.There were 30 differential substances.According to the metabolite Log2FC,there were 6 components with large difference ratio,which were polymethyl flavonoid derivatives,isorhamnoside and rhamnoside,marigold glycoside,geranylside and chrysanthin.In red beet root,5 kinds of differential metabolites content were higher than those in sugar beet roots,and 25 kinds of differential metabolites content was lower than those in sugar beet root.Compared with sugar beet root,the components with higher content of flavone metabolites in red beet root were vitexin,isovitexin glucoside and apigenin glucoside.In the biosynthetic pathways of flavonoids and flavonols,seven substances were annotated to the KEGG pathway.【Conclusion】There were significant differences of flavonoids in two kinds of beet materials,which could be used as characteristic substances for further analysis.