牙槽骨骨髓间充质干细胞膜片复合PCL/HA支架具有良好早期成骨效果:基于动物模型研究

Alveolar bone marrow mesenchymal stem cells sheet combined with PCL/HA scaffold displays better osteogenesis effect in the early healing process:based on animal models

目的评估牙槽骨骨髓间充质干细胞膜片复合PCL/HA支架的骨缺损再生修复效果。方法培养牙槽骨骨髓间充质干细胞(Al-BMSCs)膜片和长骨骨髓间充质干细胞(Lon-BMSCs)膜片,熔融沉积成型技术制作PCL/HA支架。取新西兰大白兔共9只,建立双侧下颌骨缺损模型,按植入材料将其分为PCL/HA支架组(A组)、Lon-BMSCs膜片复合PCL/HA支架组(B组)、Al-BMSCs膜片复合PCL/HA支架组(C组),3组共得到18个样本,6个/组。术后4周处死动物,取下颌骨组织,行大体观察、锥形束CT分析、HE染色和Masson染色,对各组成骨情况进行分析。结果锥形束CT结果显示C组骨体积分数、骨小梁厚度和骨小梁数量均高于A组和B组,组间差异有统计学意义(P<0.05)。HE和Masson染色显示C组成骨最为活跃,有较多的新生骨和血管形成。结论牙槽骨骨髓间充质干细胞膜片复合PCL/HA支架具有良好的早期成骨效果,为颌面部骨缺损再生修复提供了一种新的策略。

Objective To investigate the repairing effect of alveolar bone marrow mesenchymal stem cells(Al-BMSCs)sheet combined with PCL/HA scaffold in rabbit mandibular defects.Methods Al-BMSCs sheets and Long bone marrow mesenchymal stem cells(Lon-BMSCs)sheets were cultured,PCL/HA scaffolds were produced by the fused deposition modeling method.Nine New Zealand rabbits were used to establish bilateral mandibular defect models.According to the implant materials,the rabbits were divided into 3 groups:Group A(PCL/HA scaffold),group B(Lon-BMSCs sheets combined with PCL/HA scaffold),group C(Al-BMSCs sheets combined with PCL/HA scaffold).A total of 18 samples were harvested,with 6 samples per group.All the experimental animals were sacrificed after 4 weeks.Gross observation,cone beam CT analysis,HE staining and Masson staining were used to evaluate the effect of bone regeneration.Results The results of cone beam CT showed that the value of BV/TV,Tb.Th,Tb.N in the group C were higher than that in group A and group B,and the difference was statistically significant(P<0.05).HE staining and Masson staining results showed that the osteogenesis in the group C was the most active,characterized by more newly formed bone and capillaries.Conclusion Al-BMSCs sheets combined with PCL/HA scaffold displays better osteogenesis effect in the early healing process,which is expected to become a new strategy for the regeneration of maxillofacial bone defects.