摘要
本研究对从山东威海双岛林场采集的松树枯萎样本进行分离培养、单孢纯化、致病性测定,得到一株能引起松树赤枯病的病原菌,编号为RCBHL。经形态学观察和多基因(ITS、TEF-1、TUB2)系统发育分析,将菌株鉴定为玫瑰拟盘多毛孢菌(Pestalotiopsis rosea)。生物学特性测定结果表明,该菌株最适生长温度为20~25℃,最优生长培养基为PDA培养基,最适生长pH值为6.0,最适光照条件为24 h光照。该研究结果可为松树赤枯病的防治提供理论依据。
In this experiment,the samples of pine needle blight were collected from the Shuangdao For⁃est Farm in Weihai,Shandong Province,and a pathogenetic strain RCBHL was obtained after separation and culture,single spore purification and pathogenicity identification test.According to morphological characteris⁃tics and multilocus phylogenetic(ITS,TEF⁃1 and TUB2)analyses,it was identified as Pestalotiopsis rosea.The testing results of biological characteristics showed that the optimum cultural conditions for P.rosea RCHBL included 20~25℃,6.0 of pH value,PDA culture medium and 24 h light.The research results would provide the theoretical basis for prevention and control of pine needle blight.
作者
韩凤英
亓玉昆
杨慧
秦永梅
周丽颜
李星仪
李海燕
刘敏
王清海
Han Fengying;Qi Yukun;Yang Hui;Qin Yongmei;Zhou Liyan;Li Xingyi;Li Haiyan;Liu Min;Wang Qinghai(Shandong Agriculture and Engineering University,Jinan 250100,China;Shandong Academy of Forestry,Jinan 250014,China;Jinan Park Development Service Center,Jinan 250001,China)
出处
《山东农业科学》
北大核心
2024年第6期113-119,共7页
Shandong Agricultural Sciences
基金
中央林业改革发展资金防灾减灾项目(鲁财资环指[2021]27号)
2022年山东省本科教学改革研究项目(Z2022211)
山东省“黄河流域生态保护和教育高质量发展”教育教学专项课题(2022HHZX005)
关键词
松树赤枯病
分离纯化
病原鉴定
生物学特性
Pine needle blight
Separation and purification
Pathogen identification
Biological charac⁃teristics
