4株双歧杆菌对秀丽隐杆线虫的体内抗衰老作用

Anti-aging Effects of Four Bifidobacterium Species on Caenorhabditis elegans

为探究4株双歧杆菌(假小链双歧杆菌BP-1、长双歧杆菌BL-3、动物双歧杆菌BA-5、婴儿双歧杆菌BI-38)对秀丽隐杆线虫的体内抗衰老作用,测定4株双歧杆菌对线虫寿命、生殖能力、活力、体形、抗逆性、体内脂褐素水平、抗氧化酶活力和丙二醛含量的影响。结果表明,与空白对照组(大肠杆菌OP50)相比,BA-5、BI-38有效地延长了线虫的寿命(P<0.05),BP-1、BL-3对线虫寿命影响不显著(P>0.05);同时BA-5、BI-38并不会减少线虫的产卵量和削弱线虫的运动能力(P>0.05),且能有效增强其吞咽能力并显著减小体形(P<0.05);BA-5、BI-38能够有效增强线虫的抗热应激和抗氧化应激能力进而延长其寿命(P<0.05),显著减少体内脂褐素积累和降低丙二醛含量(P<0.05),并显著提高线虫体内的2种抗氧化酶(过氧化氢酶、超氧化物歧化酶)的活力(P<0.05)。综上,婴儿双歧杆菌BI-38和动物双歧杆菌BA-5具有更强的体内抗衰老活性。

The anti-aging effects of four Bifidobacterium species(B.pseudospermum BP-1,B.longum BL-3,B.animalis BA-5 and B.infantis BI-38)on Caenorhabditis elegans were evaluated in terms of its lifespan,reproductive capacity,vitality,body shape,stress resistance,lipofuscin levels,antioxidant enzyme activity and malondialdehyde(MDA)content.The results showed that compared with the control group(Escherichia coli OP50),BA-5 and BI-38 effectively extended the lifespan of C.elegans(P<0.05),while BP-1 and BL-3 had no significant effect on the lifespan of C.elegans(P>0.05).Meanwhile,BA-5 and BI-38 did not impair the reproductive or locomotory capacities of C.elegans(P>0.05),but could effectively enhance its pharyngeal pumping and reduce its body size(P<0.05).BA-5 and BI-38 were able to enhance tolerance to heat or oxidative stress and thus prolong the lifespan of C.elegans(P<0.05),significantly reduce lipofuscin accumulation and MDA content(P<0.05),and significantly increase the activity of two antioxidant enzymes(catalase and superoxide dismutase)in C.elegans(P<0.05).In conclusion,B.infantis BI-38 and B.animalis BA-5 had stronger anti-aging activity in vivo.