Fe_(3)O_(4)纳米粒子的制备及载药释放性能研究

Preparation of Fe_(3)O_(4) nanoparticles and its drug-loading and drug-release properties

采用改进的Hummers法获得氧化石墨烯(GO),通过共沉淀法制备了GO与磁性Fe_(3)O_(4)纳米粒子结合的GO/Fe_(3)O_(4)复合材料,分析了复合材料的晶体结构、微观形貌、磁化性能和载药性能,研究结果表明:GO/Fe_(3)O_(4)复合材料具有较高的结晶度,GO掺杂改善了Fe_(3)O_(4)颗粒的均匀度,晶粒尺寸为13~15 nm。GO/Fe_(3)O_(4)复合材料表现出超顺磁性,饱和磁化强度有轻微降低。利用GO/Fe_(3)O_(4)负载阿霉素(DOX)研究了载药释放性能,GO/Fe_(3)O_(4)/DOX包合物的DOX包封率均值为62.24%,具有较高的包封率,药物释放具有一定的pH依赖性,在pH=7.4时,GO/Fe_(3)O_(4)的累计释放率在75 h达到73.3%,摄入试验测试表明GO/Fe_(3)O_(4)载体具有一定的靶向性,在生物医学领域有广阔的应用前景。

Graphene oxide(GO)was obtained by the improved Hummers method.The GO/Fe_(3)O_(4) composite,in which GO was combined with magnetic Fe_(3)O_(4) nanoparticles,was prepared by co-precipitation method.The crystalline structure,microscopic morphology,magnetic properties and drug loading of the composite were analyzed.The results showed that:The GO/Fe_(3)O_(4) composite had high crystallinity;GO doping improved the uniformity of Fe_(3)O_(4) particles,and the grain size of GO/Fe_(3)O_(4) composite was 13-15 nm.The GO/Fe_(3)O_(4) composite exhibited superparamagnetic properties with a slight decrease in saturation magnetization.The drug-release properties were studied by using GO/Fe_(3)O_(4) composite to load doxorubicin(DOX).The average encapsulation efficiency of GO/Fe_(3)O_(4)/DOX inclusion complex was 62.24%,which was quite high.The drug release showed pH dependence.When the pH was 7.4,the cumulative release rate reached 73.3%in 75 h.The cellular uptake test showed that the GO/Fe_(3)O_(4) carrier had certain targeting properties and could be used as a good new drug carrier.