麝香草酚三甲氧基肉桂酸酯抑制黑色素生成、转移及AGEs合成的功效研究

Efficacy of thymol trimethoxycinnamate on melanin production,transfer and AGEs synthesis

基于皮肤细胞模型研究了麝香草酚三甲氧基肉桂酸酯的美白功效,采用CCK-8法和观察细胞状态检测麝香草酚三甲氧基肉桂酸酯对B16-F10细胞活率的影响;分别采用NaOH裂解法和多巴氧化法分析B16-F10细胞中黑色素含量和酪氨酸酶活性变化;采用细胞划痕法和Transwell小室法测定B16-F10细胞迁移能力变化;利用荧光分析法检测体外糖化实验中荧光性AGEs含量变化。结果表明麝香草酚三甲氧基肉桂酸酯质量浓度在10μg/mL及以下时对细胞活率没有明显影响,并且在10μg/mL时对黑色素合成、酪氨酸酶活性以及细胞迁移能力抑制率最高;200μg/mL时在体外对荧光性AGEs生成的抑制率达到97.75%。研究结果表明麝香草酚三甲氧基肉桂酸酯可以明显抑制黑色素的生成并抑制酪氨酸酶的活性,具有较好的美白功效;由于其同时具有较好的抗糖化能力,可作为潜在的抗衰老原料。

The whitening effect of thymol trimethoxycinnamate was studied based on skin cell model.CCK-8 method and cell state observation were used to detect the effect of thymol trimethoxycinnamate on the viability of B16-F10 cells.NaOH lysis method and dopa oxidation method were used to analyze the changes of melanin content and tyrosinase activity in B16-F10 cells.Cell scratch method and Transwell chamber method were used to determine the migration ability of B16-F10 cells.Fluorescence analysis was used to detect the changes of fluorescent AGEs content in the saccharification experiment in vitro.The results show that thymol trimethoxycinnamate has no significant effect on cell viability at or below 10μg/mL,and has the highest inhibition rate on melanin synthesis,tyrosinase activity and cell migration at 10μg/mL.The inhibition rate of fluorescent AGEs production in vitro is 97.75%at 200μg/mL.The results show that thymol trimethoxycinnamate can obviously inhibit the formation of melanin and tyrosinase activity,and has a good whitening effect.Because of its good anti-saccharification ability,it can be used as a potential anti-aging material.