基于网络药理学分析赖氨肌醇维B_(12)口服溶液治疗小儿厌食症的作用机制

Mechanism of lysine inositol vitamin B_(12) oral solution in the treatment of child anorexia based on network pharmacology

目的基于网络药理学探讨赖氨肌醇维B_(12)口服溶液治疗小儿厌食症的作用机制。方法通过查阅说明书,挖掘赖氨肌醇维B_(12)口服溶液的成分,并通过Swiss Target Prediction网站预测成分的相关靶点,与厌食症靶点进行交集映射,获得其治疗小儿厌食症的作用靶点。利用蛋白互作筛选核心靶点,并进行分子对接验证。基于共有靶点,采用功能注释生物信息学芯片进行分析,得到赖氨肌醇维B_(12)口服溶液治疗小儿厌食症的关键通路。结果赖氨肌醇维B_(12)口服溶液中的主要成分为盐酸赖氨酸、肌醇和维生素B_(12),共预测出40个靶基因,其中有13个靶基因与厌食症极为密切,主要涉及谷氨酸受体信号通路、神经活性配体-受体相互作用等相关分子。结论赖氨肌醇维B_(12)口服溶液可能发挥类似于谷氨酸的作用,通过激活突触后膜的谷氨酸受体来改善小儿的厌食症,体现了多成分、多靶点协同起效的作用特点。

Objective To explore the mechanism of Lysine Inositol and Vitamin B_(12) Oral Solution in the treatment of children with anorexia based on network pharmacology.Methods By referring to the manual,the components of lysine inositol and vitamin B_(12) oral solution were excavated,and the correlation targets of compo-nents were predicted through Swiss Target Prediction website,and the intersection mapping was performed with the targets of anorexia,so as to obtain the therapeutic targets of pediatric anorexia.Protein interaction was used to screen the core targets,and molecular docking verification was carried out.Based on the common targets,functional annotation bioinformatics chip was used to analyze,and the key pathway of Lysine Inositol and Vitamin B_(12) oral solution in the treatment of pediatric anorexia was obtained.Results Lysine hydrochloride,inositol vitamin B_(12) were the main components in lysine inositol and vitamin B_(12) oral solution.A total of 40 target genes were predicted,among which 13 target genes were closely related to anorexism,mainly involving glutamate receptor signaling pathway,neuroactive ligand receptor interaction and other related molecular interactions.Conclusion lysine inositol and vitamin B_(12) oral solution may play a similar role to glutamate,and improve child anorexia by activating glutamate receptors in the postsynaptic membrane,which reflects the characteristics of multi-component and multi-target synergistic action.