红大戟水提物的急性毒性及抗炎、镇痛、抗凝血作用研究

Study on Acute Toxicity,Anti-inflammatory,Analgesic,and Anticoagulant of the Water Extract of Knoxia valerianoides

目的:对红大戟水提物的急性毒性及抗炎、镇痛、抗凝血作用进行研究。方法:采用最大给药量法对小鼠进行急性毒性试验,用二甲苯致小鼠耳肿胀试验、脂多糖(LPS)诱导炎症小鼠模型评价其抗炎作用,用小鼠热板试验和醋酸扭体试验评价其镇痛作用。通过检测凝血酶原时间(PT)、活化部分凝血活酶时间(APTT)、凝血酶时间(TT)评价其抗凝血作用。结果:红大戟水提物最大给药量(120 g/kg/d)灌胃后连续观察14 d,全部小鼠均未出现死亡和明显的急性毒性反应。在抗炎研究中,红大戟水提物高、中、低剂量均能显著降低小鼠耳肿胀度(P<0.05或P<0.01),肿胀抑制率分别为39.5%、28.6%和20.2%,且能显著降低炎症模型小鼠血清中IL-6和TNF-α含量(P<0.05或P<0.01),而对IL-1β无显著性影响。在镇痛研究中,红大戟水提物各剂量在120 min时均能显著提高小鼠的热板痛阈值(P<0.01),且能显著降低小鼠扭体次数(P<0.05或P<0.01),扭体抑制率分别为36.7%、29.3%和23.5%。抗凝研究中,红大戟水提物高剂量能显著延长小鼠血浆PT值(P<0.05),而对APTT、TT无显著性影响。结论:红大戟水提物在最大给药量(120g/kg/d)条件下无明显的急性毒性反应,其具有明显的抗炎镇痛抗凝血作用。

Objective To study the acute toxicity,anti-inflammatory,analgesic and anticoagulant effects of the water extract of Knoxia valerianoides.Methods Mice were given the maximum dose of Knoxia valerianoides to observe the acute toxicity.The anti-inflammatory were studied by xylene-induced ear swelling and an inflammation model of lipid polysaccharides(LPS)induced mice.Analgesic effects were studied by hot plate test and glacial acetic acid-induced writhing text.The anticoagulant effect was evaluated by the prothrombin time(PT),activated partial prothrombin time(APTT),thrombin time(TT).Results At the maximum dose of the water extract of Knoxia valerianoides,no death or acute toxicity was observed within 14 days in the mice.At the anti-inflammatory study,all dose of the water extract of Knoxia valerianoides had inhibitory effects on xylene induced auricle inflammation(P<0.05 or P<0.01),the inhibition rates were 39.5%,28.6%and 20.2%.It could significantly reduce the contents of IL-6 and TNF-α(P<0.05 or P<0.01),but had no significant effect on IL-1β.At the analgesic study.All dose of the water extract of Knoxia valerianoides had inhibitory effects on acetic acid-induced writhing in mice(P<0.05 or P<0.01),the inhibition rates were 36.7%,29.3%and 23.5%.The pain threshold of mice could be significantly prolonged(P<0.01).At the anticoagulant study,the high dose of the water extract of Knoxia valerianoides could significantly prolonged the prothrombin time(P<0.05).Conclusion Knoxia valerianoides has no obvious acute toxicity at the maximum dose of 120 g/kg/d.It has anti-inflammatory,analgesic and anticoagulant effects.