SGLT-2抑制剂对糖尿病小鼠血管内皮细胞的影响

Effects of SGLT-2 Inhibitor on Vascular Endothelial Cells in Diabetes Mice

【目的】探讨钠-葡萄糖协同转运蛋白2(SGLT-2)抑制剂对糖尿病小白鼠血管内皮细胞的影响。【方法】将24只SPF级8周龄雄性ICR小白鼠腹腔注射低剂量链脲佐菌素(STZ)(50 mg/kg),建立糖尿病模型,造模成功,随机分为模型组和治疗组(达格列净治疗12周),6只小白鼠作为对照组,皮下注射生理盐水。三组均普通饲料喂养,动态观察每周小鼠体重、血糖的变化,比较三组小白鼠血浆肿瘤坏死因子-α(TNF-α)、白介素-1β(IL-1β)、单核细胞趋化因子-1(MCP-1)、超氧化物歧化酶(SOD)、丙二醛(MDA)水平。【结果】第2周末至第12周末,模型组、治疗组体重均低于对照组,治疗组体重均高于模型组,差异有统计学意义(P<0.05)。与对照组比较,模型组、治疗组血糖显著升高,差异有统计学意义(P<0.05);与模型组比较,3周末至12周末,治疗组小白鼠血糖水平显著下降,差异有统计学意义(P<0.05)。模型组血浆TNF-α、IL-1β、MCP-1水平显著高于对照组,治疗组血浆TNF-α、IL-1β、MCP-1水平显著低于模型组,差异有统计学意义(P<0.05)。模型组血浆SOD水平低于对照组,治疗组血浆SOD水平高于模型组,差异有统计学意义(P<0.05);与对照组比较,模型组血浆MAD水平高于对照组,治疗组血浆MAD水平低于模型组,差异有统计学意义(P<0.05)。【结论】SGLT-2抑制剂能有效抑制糖尿病小鼠血管内皮细胞炎症因子的释放,改善血管内皮细胞功能。

【Objective】To investigate the effect of sodium glucose cotransporter 2(SGLT-2)inhibitor on vascular endothelial cells in diabetes mice.【Methods】Twenty four SPF 8-week old male ICR mice were intraperitoneally injected with low-dose streptozotocin(STZ)(50 mg/kg)to establish a diabetes model.The model was successfully established.They were randomly divided into a model group and a treatment group(Daggligin intervention treatment for 12 weeks).Six mice were used as the control group,and physiological saline was injected subcutaneously.Three groups were all fed with regular feed,and the changes in body weight and blood glucose of mice were dynamically observed every week.The plasma levels of tumor necrosis factor alpha(TNF-α),interleukin-1β(IL-1β),monocyte chemotactic factor-1(MCP-1),superoxide dismutase(SOD),and malondialdehyde(MDA)were compared among the three groups of mice.【Results】From the 2nd weekend to the 12th weekend,both the model group and the treatment group had lower body weight than the control group,while the treatment group had higher body weight than the model group,with a statistically significant difference(P<0.05).Compared with the control group,the blood glucose levels in the model group and treatment group were significantly increased,and the difference was statistically significant(P<0.05);Compared with the model group,the blood glucose levels of the treatment group mice significantly decreased from the 3rd to the 12th weekend,and the difference was statistically significant(P<0.05).The plasma levels of TNF-α,IL-1β,and MCP-1 in the model group were significantly higher than those in the control group,while the plasma levels of TNF-α,IL-β,and MCP-1 in the treatment group were significantly lower than those in the model group,with statistical significance(P<0.05).The plasma SOD level in the model group was lower than that in the control group,while the plasma SOD level in the treatment group was higher than that in the model group,with a statistically significant difference(P<0.05);Compared with the control group,the plasma MAD level in the model group was higher than that in the control group,while the plasma MAD level in the treatment group was lower than that in the model group,with a statistically significant difference(P<0.05).【Conclusion】SGLT-2 inhibitor can effectively inhibit the release of inflammatory factors from vascular endothelial cells in diabetes mice,and improve the function of vascular endothelial cells.