黄芩苷对单增李斯特菌诱导巨噬细胞炎性小体激活的抑制作用

Inhibition of baicalin on macrophage inflammasomeactivation induced by Listeria monocytogenes

为研究黄芩苷对单增李斯特菌(Listeria monocytogenes,LM)活化的小鼠巨噬细胞炎性小体的影响,用四唑盐(methylthiazolyldiphenyl-tetrazolium bromide,MTT)法确定黄芩苷最适质量浓度梯度,选用25、50、100μg/mL的黄芩苷分别与LM处理巨噬细胞,检测细胞炎性小体相关蛋白或mRNA表达水平以及乳酸脱氢酶(lactate dehydrogenase,LDH)释放量.结果显示:黄芩苷剂量依赖性地下调了LM处理后的IL-1β、caspase-1 p10蛋白水平,LDH的释放量以及NLRP3、NLRC4、NLRP10、NOD、AIM2、caspase-1、IL-1β、IL-18的mRNA水平.因此推测,黄芩苷可以通过抑制LM诱导巨噬细胞内不同炎性小体的激活,进而抑制巨噬细胞炎症因子的释放及细胞死亡.

In order to study the effect of baicalin on the inflammasome of mouse macrophages activated by Listeria monocytogenes(LM),the optimal concentration gradient of baicalin was determined by methylthiazolyldiphenyl-tetrazolium bromide(MTT)method.Then macrophages were treated with LM and baicalin at concentrations of 25,50 and 100μg/mL,respectively,to detect the expression level of inflammasome-related protein or mRNA and lactate dehydrogenase(LDH)release.The results showed that baicalin reduced IL-1β,caspase-1 p10 protein levels,LDH release and mRNA levels of NLRP3,NLRC4,NLRP10,NOD,AIM2,caspase-1,IL-1βand IL-18 after LM treatment in a dose-dependent manner.Therefore,it is speculated that baicalin can inhibit the release of inflammatory factors and cell death in macrophages by inhibiting the activation of different inflammasome in macrophages induced by LM.