小鼠胚胎玻璃化冷冻自制载体的效果分析

Effectiveness of a newly self-made carrier for cryopreservation of mouse embryos

目的:研究自制的冷冻载体是否可以冷冻保存ICR小鼠卵裂胚和囊胚。方法:比较自制载体Cryotip和商业化载体Cryotop开展小鼠卵裂胚胎玻璃化冷冻效果,以及玻璃化冷冻保存小鼠囊胚的结局;对Cryotip组和Cryotop组卵裂胚胎复苏率、囊胚形成率、囊胚复苏率以及孵化率进行数据分析。结果:Cryotip组和Cryotop组冷冻小鼠卵裂胚胎的复苏率和囊胚形成率、囊胚孵化率差异均无统计学意义。在小鼠囊胚冻存方面,复苏率和囊胚孵化率差异无统计学意义。结论:自制载体Cryotip具有与商业化载体Cryotop接近的冷冻保存ICR小鼠卵裂胚胎和囊胚的效果。

Objective:To investigate the feasibility of cryopreservation of ICR mouse cleaved embryos and blastocysts with our self-made carrier.Methods:The vitrification effect of mouse cleavage embryos and blastocyst were compared between our self-made Cryotip and commercial Cryotop.The data of cleavage embryos recovery rate,blastocyst formation rate,blastocyst recovery rate and hatching rate in the Cryotip grou p and the Cryotop group were analyzed.Results:There was no significant difference in the cleavage embryos recovery rate,blastocyst formation rate and blastocyst hatching rate of mice cryopreserved in Cryotip and commercial Cryotop groups.The blastocyst hatching rate,recovery rate,and blastocyst formation rate were slightly lower in the Cryotip group compared with the Cryotop group.There was no significant difference in the recovery rate and blastocyst hatching rate in mouse blastocyst cryopreservation.Conclusion:Our self-made carrier Cryotip shows close effect in cryopreservation of ICR mouse cleavage embryos and blastocysts that are comparable to the commercial carrier Cryotop.