温郁金根茎腐烂病的病原鉴定

Pathogen identification of root rot of Curcuma wenyujin

在浙江省瑞安市陶山镇温郁金主产区,部分田块温郁金发生大规模倒伏、地下根茎严重腐烂。为明确引起该病害的病原物,利用LB培养基和TTC(2,3,5-氯化三苯基四氮唑)培养基从温郁金腐烂根茎中分离获得细菌菌株JF1,采用灌根法、芽侵染法和根茎侵染法确定该菌株对温郁金的致病性。通过形态学特征观察、生理生化测定、多基因序列(16S rDNA、rpoB、gyrB)系统发育分析,明确引起田间温郁金根茎腐烂的病原菌为桑肠杆菌(Enterobacter mori)。

At main production areas of Curcuma wenyujin in Taoshan Town,Rui an City,Zhejiang Province,large-scale lodging of C.wenyujin and severe rot of their underground tubers occurred in some fields.To identify the pathogen that causes the disease,the bacteria strain JF1 was isolated from the rotten tuber of C.wenyujin by using LB and TTC(2,3,5-triphenyltetrazolium chloride)medium.The pathogenicity of isolated strain JF1 was confirmed by using three inoculation methods,including root irrigation,bud infection and rhizome infection.The taxonomic status of pathogenic bacteria was identified according to the morphological characteristics,physiological and biochemical tests,phylogenetic analysis of multiple gene sequences(16S rDNA,rpoB,gyrB).The results showed that the pathogenic bacteria causing root rot of C.wenyujin was Enterobacter mori.