宫颈癌组织中EMC6、C-myc及BCL-2的表达和预后

Expression of EMC6,C-myc and BCL-2 in Cervical Cancer Tissues and Prognosis Analysis

目的通过生信分析,评估EMC6基因在泛癌及宫颈癌中的表达、免疫相关性及诊断价值,并探究EMC6、C-myc、BCL-2蛋白与宫颈癌的相关性及预后。方法分析TCGA和GTEx数据库中EMC6在泛癌和宫颈癌的表达,并利用SangerBox和Timer数据库评估EMC6与免疫相关性,以及ROC评估诊断潜力。通过免疫组化在68例宫颈癌及癌旁组织中检测EMC6、C-myc、BCL-2蛋白表达,分析其与临床病理特征的联系。使用Phi系数法分析相关性,并利用Kaplan-Meier法评估这些蛋白对预后的影响。结果宫颈癌中EMC6表达降低,对免疫浸润有显著影响,且诊断准确性较高(AUC=0.819)。免疫组化显示,与宫颈癌旁组织相比,EMC6蛋白表达下降、而C-myc和BCL-2蛋白表达上调,差异均有统计学意义(P<0.05)。EMC6表达与临床病理特征无相关性(P>0.05),而C-myc与病理类型相关(P<0.05),BCL-2与年龄、病理类型、肿瘤大小、分化程度相关(P<0.05)。EMC6与C-myc、BCL-2表达在宫颈癌中呈负相关(Phi=-0.367,P=0.002;Phi=-0.284,P=0.019),且三者与患者总生存期显著相关(P<0.05)。结论宫颈癌中EMC6蛋白表达下降,与C-myc、BCL-2蛋白表达呈负相关,且这些蛋白的表达水平与患者的总生存期之间存在显著的相关性,提示它们可能作为宫颈癌治疗和预后评估的潜在生物标志物。

Objective This study employs bioinformatics analysis to evaluate the expression,immune correlation,and diagnostic value of EMC6 gene in pan-cancer and cervical cancer,and investigate the associations of EMC6,C-myc,and BCL-2 proteins with cervical cancer and patient prognosis.Methods This study analyzed the expression of EMC6 across pan-cancer and cervical cancer datasets from TCGA and GTEx,utilizing SangerBox and Timer databases to assess its correlation with immune response and employing ROC analysis to evaluate its diagnostic potential.EMC6,C-myc,and BCL-2 protein expressions were detected in 68 cases of cervical cancer and adjacent tissues using immunohistochemistry,and their associations with clinicopathological characteristics were analyzed.The Phi coefficient method was employed to assess correlations,and the Kaplan-Meier method was used to evaluate the impact of these proteins on prognosis.Results In cervical cancer,the expression of EMC6 decreased,which significantly affected immune infiltration and had a high diagnostic accuracy(AUC=0.819).Immunohistochemistry revealed that compared to the adjacent tissues of cervical cancer,the expression of EMC6 protein decreased,while the expressions of C-myc and BCL-2 proteins were upregulated,with statistically significant differences(P<0.05).EMC6 expression showed no correlation with clinicopathological features(P>0.05),while C-myc was associated with pathological type(P<0.05),and BCL-2 was associated with age,pathological type,tumor size,and degree of differentiation(P<0.05).In cervical cancer,the expression of EMC6 was negatively correlated with C-myc and BCL-2(Phi=-0.367,P=0.002;Phi=-0.284,P=0.019),and all three were significantly associated with overall patient survival(P<0.05).Conclusions The expression of EMC6 protein decreases in cervical cancer and is negatively correlated with the expression of C-myc and BCL-2 proteins.There is a significant correlation between the expression levels of these proteins and the overall survival of patients,suggesting that they may be used as treatments for cervical cancer and Potential biomarkers for prognostic assessment.