宁夏刺槐叶瘿蚊不同阶段幼虫及蛹的抗寒性研究

Study on the Cold Resistance of Larvae and Pupae of Obolodiplosis robiniae in Ningxia

为研究刺槐叶瘿蚊在宁夏是否可以越冬,同时也为其综合防治提供理论依据,在室外采集刺槐叶瘿蚊虫体,带回实验室后划分为低龄幼虫、老熟幼虫、蛹,测定不同虫态的过冷却点和结冰点。结果表明:刺槐叶瘿蚊同一虫龄不同重复间的过冷却点无显著差异。低龄幼虫、老熟幼虫、蛹之间的过冷却点和结冰点的差异明显,其中蛹的数值最高,过冷却点和结冰点的平均温度分别为-23.24、-20.13℃;低龄幼虫的数值最低,过冷却点和结冰点的平均温度分别为-27.19、-25.16℃。根据测定结果,刺槐叶瘿蚊抗寒性最强的虫态为低龄幼虫,其次为老熟幼虫,最后为蛹。结合宁夏冬季气温得出,刺槐叶瘿蚊在宁夏地区可以越冬,最有可能的越冬虫态为低龄幼虫。

The purpose of this study was to investigate whether Obolodiplosis robiniae has the ability to overwinter in Ningxia and to provide a theoretical basis for its integrated pest management.Specimens of O.robiniae were collected outdoors and divided into low-instar larvae,mature larvae,and pupae.The supercooling points and freezing points of these different developmental stages were then determined in the laboratory.Results showed no significant difference in the supercooling points among different replicates of the same developmental stage.However,significant differences were observed among the different stages.Pupae exhibited the highest supercooling and freezing points,with average temperatures of-23.24℃and-20.13℃,respectively.Lowinstar larvae had the lowest values,with average temperatures of-27.19℃for the supercooling point and-25.16℃for the freezing point.Based on these results,low-instar larvae demonstrated the strongest cold resistance,followed by mature larvae and pupae.