宁夏沙生药用植物AMF、DSE侵染特征及DSE鉴定

Characteristics of AMF and DSE Colonization of Ningxia Sand-Grown Medicinal Plants and Identification of DSE

为研究宁夏沙生药用植物根系丛枝菌根真菌(arbuscular mycorrhizal fungi,AMF)与深色有隔内生真菌(dark septate endophytes,DSE)的侵染特征及DSE物种多样性,在宁夏各地采集健康的黄耆、苦豆子和柠条等13种沙生药用植物的根样,显微观察AMF和DSE的侵染特征,采用形态学结合分子生物学鉴定,研究DSE物种多样性。结果表明:13种植物根系中均有AMF定植;除乳浆大戟外12种植物根系均有DSE定植;AMF定植率高于DSE定植率。在苦豆子、沙蒿、沙冬青、黄耆、柠条5种植物根系分离得到4株DSE菌株,分别是Cladosporium cladosporioides、Alternaria sp.、Aspergillus fumigatus、Paraphoma chrysanthemicola。

To study the colonization characteristics of AMF(arbuscular mycorrhizal fungi)and DSE(dark septate endophytes)in the roots of sand-grown medicinal plants in Ningxia,as well as the diversity of DSE species,root samples from 13 healthy plant species including Astragalus membranaceus(Fisch.)Bunge,Sophora alopecuroides Linn,and Caragana korshinskii were collected in Ningxia.Microscopic observation was used to investigate the colonization characteristics of AMF and DSE.Morphological and molecular biology techniques were applied to study the diversity of DSE species.The results show that all 13 sand-grown medicinal plants were colonized by AMF;12 of the plants,except Euphorbia esula Linn,were colonized by DSE;and the colonization rate of AMF was higher than that of DSE.Four strains of DSE were isolated from 5 plant species,which were identified as Cladosporium cladosporioides,Alternaria sp.,Aspergillus fumigatus,and Paraphoma chrysanthemicola.