万丈深提取物对脾虚模型小鼠健脾消食作用研究

Spleen-tonifying and Digestion-promoting Effect of Crepis phoenix Dunn Extract on Mice Model of Spleen Deficiency

目的:制备小鼠脾虚模型,探讨万丈深Crepis phoenix Dunn.提取物的健脾消食作用。方法:将实验动物随机分为空白组、模型组、阳性对照(参苓健脾胃颗粒10.3 g·kg^(-1))组、万丈深水提物(1.0、2.0、4.0 g·kg^(-1))剂量组、万丈深醇提物(1.0、2.0、4.0 g·kg^(-1))剂量组,除空白组外,其余各组小鼠采用大黄联合番泻叶灌胃诱导小鼠脾虚证候模型。造模成功后,各组小鼠以20 mL·kg^(-1)灌胃给予相应样品,1次/d,连续7 d。以小鼠体质量、自主活动量、体温、食物利用率、脾脏指数、胃排空率、小肠推进率、胃肠激素等为指标,评价万丈深的健脾消食作用。结果:与空白组比较,模型组小鼠体质量增长量、食物利用率、体温、自主活动量、血清胃动素(MTL)水平、血清胃泌素(GAS)水平、胃排空率、脾脏指数降低,血清血管活性肠肽(VIP)水平、小肠推进比升高,表明小鼠脾虚证候模型制备成功。与模型组比较,万丈深水提物对小鼠体质量增长量、食物利用率、体温、脾脏指数具有明显的改善作用,但对小鼠自主活动量、血清MTL水平、血清GAS水平、血清VIP水平、胃排空率、小肠推进比无明显改善;万丈深醇提物对小鼠体质量增长量、食物利用率、自主活动量、血清MTL水平、血清VIP水平、胃排空率、小肠推进比、脾脏指数具有明显的改善作用,但对小鼠体温及血清GAS水平无明显改善。结论:万丈深具有明显的健脾消食作用,且醇提物的健脾消食作用优于水提物,其机制可能与调节胃肠激素及免疫力有关。

Objective:To establish a mouse model of spleen deficiency and investigate the spleen-tonifying and digestion-promoting effects of water extract of Crepis phoenix Dunn.Methods:The experimental animals were randomly divided into blank group,model group,positive control(Shenling Jianpiwei Granules of 10.3 g·kg^(-1))group,water extract groups of C.phoenix(1.0,2.0,and 4.0 g·kg^(-1)),and ethanol extract groups of C.phoenix(1.0,2.0,and 4.0 g·kg^(-1)).Except for the blank group,the mice in other groups were administered intragastrically with rhubarb combined with senna leaf to induce the mouse model of spleen deficiency syndrome.After successful modeling,mice in each group were given the corresponding sample by gavage at 20 mL·kg^(-1),once a day for seven consecutive days.The body weight,independent activity,body temperature,food utilization rate,spleen index,gastric emptying rate,small intestine propulsion rate,gastrointestinal hormone,and other indicators were used to evaluate the spleen-tonifying and digestion-promoting effect of C.phoenix.Results:Compared with the blank group,the body weight increase,food utilization rate,body temperature,independent activity,serum motilin(MTL)level,serum gastrin(GAS)level,gastric emptying rate,and spleen index of the mice in the model group were decreased,while the serum vasoactive intestinal peptide(VIP)level and small intestine propulsion rate were increased,indicating that the mouse model of spleen deficiency syndrome was successfully established.Compared with the model group,the water extract of C.phoenix had a significant improvement in body weight increase,food utilization rate,body temperature,and spleen index in mice but slightly improved the independent activity,serum MTL level,serum GAS level,serum VIP level,gastric emptying rate,and small intestinal propulsion rate.The ethanol extract of C.phoenix had a significant improvement in body weight increase,food utilization rate,independent activity,serum MTL level,serum VIP level,gastric emptying rate,small intestinal propulsion rate,and spleen index in mice but slightly improved the body temperature and serum GAS level.Conclusion:C.phoenix has obvious spleen-tonifying and digestion-promoting effects,and the spleen-tonifying effect of the ethanol extract is better than that of the water extract.The mechanism may be related to regulating gastrointestinal hormones and immunity.