慢性阻塞性肺病急性发作期外周血lncRNA XIST和miR-124的表达水平及临床意义

Expression levels and clinical significance of lnc RNA XIST and mi R-124 in peripheral blood monocytes during acute exacerbation of chronic obstructive pulmonary disease

目的探讨慢性阻塞性肺病(COPD)急性发作期外周血单核细胞中长链非编码RNA X无活性特异性转录本(lncRNA XIST)和miR-124的表达水平及临床意义。方法选取2020年11月-2023年3月邯郸市第一医院收治的处于急性发作期的123例COPD患者作为急性期组,选择处于稳定期的121例COPD患者作为稳定期组,另选同期体检的122名健康志愿者作为对照组。检测3组白介素(IL)-1β、IL-10、IL-12、肿瘤坏死因子(TNF-α)及外周血单核细胞lncRNA XIST和miR-124表达水平;Pearson法相关性分析急性期组lncRNA XIST和miR-124的相关性;受试者工作特征(ROC)曲线分析lncRNA XIST和miR-124对COPD急性期的诊断价值;多因素logistic回归分析COPD急性发作期的影响因素。结果与对照组比较,急性期组和稳定期组IL-1β、IL-12、TNF-α、lncRNA XIST表达水平显著升高,IL-10、miR-124表达水平显著降低,差异均有统计学意义(F=58.987、95.674、106.138、237.468、45.710、126.014,P均<0.001)。Pearson法相关性分析显示,急性期组lncRNAXIST和miR-124成负相关(r=-0.519,P<0.001)。ROC曲线下面积(AUC)显示,二者联合诊断的AUC显著大于lncRNA XIST和miR-124单独诊断(Z=3.726、2.812,P均<0.05)。多因素logistic回归分析显示,lncRNA XIST和miR-124是COPD急性发作期的影响因素(P均<0.05)。结论COPD急性发作期患者外周血单核细胞内的lncRNA XIST表达水平显著上升,miR-124显著下降,二者存在负相关关系,同时也是COPD急性发作期的影响因素,二者联合检测可提高诊断效能。

Objective To explore the expression levels and clinical significant of long non-coding RNA X inactive specific transcript(lncRNA XIST) and miR-124 in peripheral blood mononuclear cells during acute exacerbation of chronic obstructive pulmonary disease(COPD). Methods A total of 123 COPD patients in the acute stage treated in the First Hospital of Handan from November 2020 to March 2023 were selected as the acute stage group;121 COPD patients in the stable stage group, and 122 healthy volunteers who underwent physical examination in our hospital during the same period were selected as the control group. The expression levels of interleukin-1β(IL-1β), IL-10, IL-12, tumor necrosis factor-α(TNF-α) and lncRNA XIST and miR-124 in peripheral blood mononuclear cells were detected in the three groups. The correlation between lncRNA XIST and miR-124 in acute stage was analyzed by Pearson method. The diagnostic value of lncRNA XIST and miR-124 in acute COPD was analyzed by receiver operating characteristic(ROC) curve. Multivariate logistic regression was used to analyze the influencing factors of acute COPD. Results Compared with the control group, IL-1β, IL-12, TNF-α and lncRNA XIST levels were significantly increased in the acute stage group and the stable stage group, and IL-10 and miR-124 expression levels were significantly decreased,the differences were statistically significant(F=58.987,95.674,106.138,237.468, 45.710, 126.014;all P<0.001). Correlation analysis by Pearson method showed that there was a significant negative correlation between lncRNA XIST and miR-124 in the acute stage group(r=-0.519,P<0.001). The area under curve(AUC) showed that the AUC of combined diagnosis was significantly larger than that of lncRNA XIST alone and miR-124 alone(Z=3.726,2.812;all P<0.05). Multiple logistic regression analysis showed that lncRNA XIST and miR-124 were influential factors in the acute exacerbation of COPD(all P<0.05). Conclusions The expression level of lncRNA XIST in peripheral blood mononuclear cells of patients with acute COPD was significantly increased, and miR-124 was significantly decreased, both of which had a negative correlation and were also influential factors in the acute COPD episode. The combined detection of both of them provided great help for the clinical diagnosis of COPD acute episode.