体质量指数对脂肪组织血管基质组分细胞亚群的影响

Effect of body mass index on the cell composition of adipose tissue stromal vascular fraction

目的 探究体质量指数(body mass index, BMI)对于脂肪组织基质血管组分(stromal vascular fraction,SVF)细胞亚群相对百分比的影响。方法 自2022年2月至2023年6月,本研究纳入61例上海交通大学医学院附属第九人民医院整复外科接受脂肪抽吸术患者的腹部皮下脂肪样本,按照BMI分为正常体质量组(BMI<25 kg/m^(2))和超重及肥胖组(BMI≥25 kg/m^(2));采用胶原酶消化法提取SVF;将获得的SVF进行流式细胞术检测;统计SVF内细胞亚群相对百分比。结果 流式细胞术检测结果显示,相对于正常体质量组,超重及肥胖组脂肪组织SVF内脂肪来源干细胞(adipose derived stem cells, ADSCs)、脂肪前体细胞、CD34^(+)细胞、内皮细胞、M2型巨噬细胞亚群相对百分比显著下降,差异有统计学意义(P<0.05);而两组间内皮祖细胞、造血干细胞、单核细胞、周细胞亚群相对百分比比较,差异无统计学意义(P>0.05);与正常体质量组相比,超重及肥胖组M1型巨噬细胞/M2型巨噬细胞比值显著上升,差异有统计学意义(P<0.05)。结论 脂肪组织SVF细胞亚群在不同BMI分组有着显著变化,超重及肥胖脂肪组织SVF的增殖、脂肪生成、血管生成和再生能力受损,炎症增加,可能进一步导致脂肪组织功能障碍,从而影响SVF临床应用的疗效。

Objective To explore the impact of body mass index(BMI)on the cell composition of the stromal vascular fraction(SVF)obtained from human subcutaneous adipose.Methods In this study,abdominal subcutaneous adipose tissue was collected from 61 patients undergoing liposuction,following which SVF was isolated for flow cytometry.Based on BMI,patients were categorized into lean groups(BMI<25 kg/m^(2))and overweight or obese groups(BMI≥25 kg/m^(2)).Results Flow cytometry results revealed significant reductions in relative percentages of ADSCs,adipose precursor cells,endothelial cells,M2 macrophages,and CD34^(+)cell subsets of adipose tissue SVF in overweight and obese groups compared to the lean group(P<0.05).However,there was no significant difference in relative percentages of endothelial progenitor cells,hematopoietic stem cells,monocytes,and pericytes between both groups(P>0.05).Notably,the M1/M2 macrophage ratio significantly increased in the overweight and obese group(P<0.05).Conclusion The cell composition of adipose tissue SVF varies significantly among different BMI groups.The proliferation,adipogenesis,angiogenesis,and regenerative functions of SVF in overweight and obese adipose tissues are impaired,which may further lead to adipose tissue dysfunction and affect the efficacy of SVF in clinical application.