西北部分地区密点麻蜥线粒体基因系统进化分析

Phylogenetic analysis of the mitochondrial genes of Eremias multiocellata in parts of Northwest China

研究旨在探讨西北地区密点麻蜥的系统进化关系,对密点麻蜥的线粒体ND2与ND4基因进行扩增并测序,基于ND2与ND4基因与国内其他麻蜥品种构建系统进化树,分析系统进化关系。结果显示,甘肃盐池湾与内蒙古鄂前旗的密点麻蜥存在基因流。基于ND2基因建立的系统进化树显示,宁夏沙坡头的密点麻蜥M88与内蒙古乌审旗的M102也存在基因流。研究表明,为保护密点麻蜥的种质资源和维护物种多样性,在制定保护措施时应避免过度的基因交流,并适当控制与近缘物种的渗透杂交。

The purpose of the study was to explore the phylogenetic relationship of the Eremiasmultiocellatain Northwest China.The mitochondrial ND2 and ND4 genes of the Eremias multiocellata were amplified and sequenced,and the phylogenetic tree was constructed based on the ND2 and ND4 genes and otherEremiasmultiocellatavarieties in China,and then the phylogenetic relationship was analyzed.The results showed that there was gene flow in Yanchiwan,Gansu,and Otog Front Banner,Inner Mongolia.The phylogenetic tree based on ND2 gene showed that gene flow was also present in Eremiasmultiocellata M88 from Shapotou,Ningxia,and M102 from Uxin Banner,Inner Mongolia.The research shows that in order to protect the germplasm resources and maintain species diversity,excessive gene exchange should be avoided when formulating protection measures,and osmotic hybridization with related species should be properly controlled.