乳样中大肠杆菌分离鉴定、药敏试验及生物膜筛选结果的分析

Analysis of E.coli isolation,identification,drug susceptibility test and biofilm screening results in milk samples

研究旨在了解南疆某奶牛场乳房炎的发病情况,并为该场奶牛乳房炎治疗用药方案提供参考。试验选择南疆某奶牛场的38个乳样进行隐性乳房炎(LMT)检测,取LMT诊断阳性样本进行细菌分离培养、药敏试验和生物膜筛选。结果显示,38份样本中15份乳样为阳性,占39.5%,23份乳样为阴性,占60.5%。对阳性样本进行细菌分离鉴定得到62株大肠杆菌,分离株对庆大霉素表现高度敏感,对壮观霉素表现耐药,且未得到阳性生物膜。研究表明,该场分理处的大肠杆菌对11种常用于治疗大肠杆菌性奶牛乳房炎的抗生素耐药性不强,可选择庆大霉素用于临床治疗。

The purpose of the study was to understand the incidence of mastitis in a dairy farm in southern Xinjiang,and to provide a reference for the treatment of mastitis in dairy cows in this farm.38 milk samples from a dairy farm in southern Xinjiang were tested for LMT diagnostic solution,and positive samples of LMT were isolated and cultured for bacteria,drug sensitivity test and biofilm screening.The results showed that among 38 samples,15 milk samples were positive,accounting for 39.5%,and 23 milk samples were negative,accounting for 60.5%.62 strains of E.coli were isolated and identified from positive samples.The strains were highly sensitive to gentamicin and resistant to spectinomycin,and no positive biofilm was obtained.The study indicated that E.coliin the field was not resistant to 11 antibiotics commonly used to treat E.colimastitis in dairy cows,so ceftazidime could be selected for clinical treatment.