7种海南特色茶抗氧化活性及总多酚、总黄酮含量的测定

Determination of Antioxidant Activity,Total Polyphenol and Total Flavonoid Content of Seven Hainan Specialty Tea

为了深入探索海南地方特色茶的抗氧化活性及总多酚、总黄酮含量的差异,本研究对7种海南特色茶(辣木茶、苦丁茶、五指山绿茶、白沙绿茶、五指山红茶、兰贵人和鹧鸪茶)提取液的抗氧化活性及总多酚、总黄酮含量进行测定。结果表明:不同品种茶叶的抗氧化活性存在差异,茶叶提取液的抗氧化活性与样品浓度均表现出量效关系,同时热稳定性较好。7种茶的总多酚和总黄酮含量分别在9.80%~22.84%和0.90%~4.67%之间,五指山绿茶的总多酚含量最高,是辣木茶的2.33倍;苦丁茶、五指山红茶、兰贵人、鹧鸪茶的总多酚含量差异不显著。五指山绿茶的总黄酮含量最高,是辣木茶的5.18倍;五指山红茶、兰贵人、鹧鸪茶总黄酮含量差异不显著,五指山绿茶的总多酚和总黄酮含量均为7种茶叶中最高。7种茶叶提取液均具有抗氧化还原能力,并且茶叶中的茶多酚含量、总黄酮含量与自由基清除能力呈现良好的量效关系,辣木茶的清除ABTS能力最强,五指山红茶的清除超氧自由基能力最强,白沙绿茶整体的抗氧化效果最好,其还原能力、DPPH清除能力、羟自由基清除能力最强。7种茶叶提取液在不同温度条件和沸水孵育,其抗氧化性并未发生显著变化,说明7种茶叶提取液的热稳定性较佳。研究表明供试7种茶不仅富含总多酚和总黄酮,而且具有较强的抗氧化活性,为海南特色茶的进一步开发利用提供理论基础。

The antioxidant activity,total polyphenol and total flavonoid content differences of local Hainan specialty teas(Moringa Tea,Kuding Tea,Wuzhishan Green Tea,Baisha Green Tea,Wuzhishan Black Tea,Languiren and Partridge Tea)were evaluated.Results revealed significant variations in antioxidant activity among the teas.The antioxidant activity of the tea extract exhibited a dose-dependent relationship with sample concentration and demonstrated excellent thermal stability.The total polyphenol and total flavonoid content of the teas was ranged from 9.80% to 22.84% and 0.90% to 4.67%,respectively,and the total polyphenol content of Wuzhishan Green Tea was the highest,which was 2.33 times that of Moringa Tea.The difference in total polyphenol content of Kuding Tea,Wuzhishan Black Tea,Languiren,and Partridge Tea was not significant,while the total flavonoid content of Wuzhishan Green Tea was the highest,and the total flavonoid content of Wuzhishan Green Tea was 5.18 times that of Moringa Tea.The difference in total flavonoid content of Wuzhishan Black Tea,Languiren,and Partridge Tea was not significant,and the total polyphenol and total flavonoid content of Wuzhishan Green Tea were the highest among the teas.The seven tea extracts had antioxidant reduction ability,and the tea polyphenol content and total flavonoid content showed a good quantitative relationship with the scavenging ability of free radicals.Moringa Tea had the strongest ability to scavenge ABTS,Wuzhishan Black Tea had the strongest ability to scavenge superoxide radicals,and Baisha Green Tea had the best antioxidant effect overall,with the reduction ability,DPPH scavenging ability,hydroxyl radical scavenging ability were the strongest.The antioxidant properties of the seven tea extracts did not change significantly when incubated at different temperature conditions and boiling water,indicating that the seven tea extracts had better thermal stability.This study shows that Hainan specialty teas are rich in total polyphenols and total flavonoids and exhibit strong antioxidant abilities,providing a theoretical foundation for further development and utilization of Hainan specialty teas.