银扇草再生体系的建立

Establishment of a Regeneration System for Lunaria annua

为建立银扇草(Lunaria annua)体外再生体系,以其真叶为外植体,探讨消毒条件、植物生长调节剂组合及浓度对愈伤组织诱导、不定芽和不定根分化的影响;进一步分析了生根方式对成苗和幼苗生长的影响。结果表明,用75%乙醇消毒45秒配合0.1%升汞溶液消毒6分钟为银扇草叶片外植体最佳消毒处理;真叶愈伤组织诱导及不定芽分化的最适培养基为MS+0.5 mg·L^(-1)6-BA+2.0 mg·L^(-1)2,4-D,愈伤组织诱导率达93.37%,不定芽分化率达84.08%;最佳生根培养基为MS+0.1 mg·L^(-1)NAA,从接种叶片外植体到获得再生植株约90天。该研究建立了银扇草稳定的再生体系,为开发利用银扇草资源及挖掘功能基因奠定了基础。

In order to establish an in vitro regeneration system for Lunaria annua,its true leaves were used as explants to study the effects of sterilization conditions,combinations and concentrations of plant growth regulator on the induction of callus and on the differentiation of adventitious buds and roots;The effect of rooting methods on the growth of seedlings and young plants was further explored.The results showed that the best disinfection treatment for leaf explants was a combination of 75%alcohol for 45 seconds and 0.1%HgCl_(2) solution for 6 minutes.The most suitable medium for induction of callus and differentiation of adventitious buds was MS+0.5 mg·L^(-1)6-BA+2.0 mg·L^(-1)2,4-D.The induction rate of callus reached 93.37%and the differentiation rate of adventitious buds reached 84.08%.The best rooting medium was MS+0.1 mg·L^(-1) NAA,and the time from inoculating leaf explants to obtaining regenerated plants was about 90 days.In this study,a stable regeneration system was established,which laid a foundation for the development and utilization of L.annua resources and digging for functional genes.