斑马鱼模型评估侧柏叶提取物的保湿功效

Zebrafish Modeling to Evaluate the Moisturizing Efficacy of Platycladus Orientalis Leaf Extracts

目的观察侧柏叶提取物的保湿功效。方法采用体外称重法测定侧柏叶提取物的保湿率;斑马鱼保湿模型进一步研究用侧柏叶提取物高中低剂量处理斑马鱼尾部后面积收缩率的变化,并与空白对照组、模型对照组以及阳性对照组相比来评估侧柏叶提取物的保湿功效。结果5%侧柏叶水提物与5%透明质酸的保湿率差异无统计学意义(P>0.05),1%侧柏叶醇提物与1%透明质酸的保湿率差异无统计学意义(P>0.05);0.2、0.4、0.8 mg·mL^(-1)侧柏叶水提物组与模型对照组相比斑马鱼尾部面积收缩率明显升高,且差异均有统计学意义(P<0.05)。结论结合体外称重法结果与斑马鱼尾部面积收缩率结果,侧柏叶水提物具有较好的保湿功效,可作为保湿原料进一步开发。

OBJECTIVE To observe the moisturizing effects of Platycladus Orientalis leaf extracts.METHODS In vitro weighing method was used to determine the moisturization rate of Platycladus Orientalis leaf extracts.The zebrafish moisturizing model was used to further investigate the changes in the area shrinkage rate of zebrafish tails treated with high,medium,and low doses of Platycladus Orientalis leaf extracts and to assess the moisturizing efficacy of Platycladus Orientalis leaf extracts in comparison with blank control,model control and positive control.RESULTS There was no statistically significant difference in moisturizing rate between 5%aqueous extract of Platycladus Orientalis leaf and 5%hyaluronic acid(P>0.05),and there was no statistically significant difference in moisturizing rate between 1%alcoholic extract of Platycladus Orientalis leaf and 1%hyaluronic acid(P>0.05).The 0.2,0.4,0.8 mg·mL^(-1) aqueous extracts of Platycladus Orientalis leaf groups showed significantly higher contraction rate of zebrafish tail area compared to the model control group,and the differences were statistically significant(P<0.05).CONCLUSION Based on the results of the in vitro weighing method and the shrinkage rate of zebrafish tail area,the aqueous extract of Platycladus Orientalis leaf has good moisturizing efficacy and can be further developed as a moisturizing raw material.