毛蕊异黄酮对脑缺血再灌注损伤大鼠的保护作用

The protective effect of calycosin on cerebral ischemia-reperfusion injury in rats

目的探讨毛蕊异黄酮对脑缺血再灌注损伤大鼠的保护作用。方法将SPF级雄性SD大鼠随机分为4组:假手术组、模型组、毛蕊异黄酮组、阳性药(依达拉奉)组。酶联免疫吸附法(ELISA)检测ANG-1、iNOS和VEGF水平;蛋白免疫印迹法(Western Blot)检测HIF-1α、EGFR和VEGFR1的蛋白表达水平;免疫组化法观察GFAP、VWF和GLUT1阳性细胞;实时荧光定量法(PCR)检测HIF-1α、VEGFA和RCAN2的mRNA表达水平。结果毛蕊异黄酮和依达拉奉均能够增加SD大鼠血清中VEGF、ANG-1和iNOS含量(P<0.05或P<0.01),增加SD大鼠脑组织中HIF-1α、VEGFR1、EGFR和GLUT1蛋白表达水平(P<0.05或P<0.01),增加SD大鼠脑组织中HIF-1α、VEGFA和RCAN2 mRNA表达量(P<0.05或P<0.01)。结论毛蕊异黄酮能够通过改善血管生成对脑缺血再灌注大鼠具有一定的保护作用,且与阳性对照药疗效相当。

Objective To study the protective effect of calycosin on cerebral ischemia-reperfusion injury in rats.Methods SPF male SD rats were randomly divided into four groups:the sham operation group,the model group,the calycosin group and the positive drug(edaravone)group.The levels of ANG-1,iNOS and VEGF were detected by enzyme-linked immunosorbent assay(ELISA).The protein expression levels of HIF-1α,EGFR and VEGFR1 were detected by Western Blot.The positive cells of GFAP,VWF and GLUT1 were observed by immunohistochemistry.The mRNA expression levels of HIF-1α,VEGFA and RCAN2 were detected by real-time fluorescence quantitative(PCR).Results Calycosin and edaravone could significantly increase the contents of VEGF,ANG-1 and iNOS in serum of SD rats(P<0.05 or P<0.01),significantly increase the protein expression levels of HIF-1α,VEGFR1,EGFR and GLUT1 in brain tissue of SD rats(P<0.05 or P<0.01),and significantly increase the mRNA expression levels of HIF-1α,VEGFA and RCAN2 in brain tissue of SD rats(P<0.05 or P<0.01).Conclusion Calycosin has a certain protective effect on cerebral ischemia-reperfusion rats by improving angiogenesis,and is equivalent to the positive control drug.