血液中6种常见毒品的GC-MS/MS定性结果评价探究

Research on GC-MS/MS qualitative result evaluation of six common drugs in blood

目的 建立并评价气相色谱-串联质谱(GC-MS/MS)检测血液基质中6种常见毒品(甲基苯丙胺、哌替啶、咖啡因、可待因、可卡因、氯胺酮)的方法,完善结果判定依据。方法 血液基质中加入上述6种毒品混标,乙醚提取后采用GC-MS/MS进行检测,多反应监测(MRM)模式下采集数据,以上述6种毒品的GC-MS/MS定性结果中保留时间和相对离子丰度比的最大允许偏差为判定依据,对定性结果进行评价。结果 6种常见毒品线性关系良好,其中氯胺酮和咖啡因的检测限最低(0.01μg/m L),甲基苯丙胺的检测限最高(0.5μg/m L)。在6个添加水平下目标物质的保留时间(RT)和相对保留时间(RRT)均比较稳定,RT的绝对偏差(ΔRT_(绝对))在±0.025 min内;RRT的绝对偏差(ΔRRT_(绝对))在±0.004内;相对离子丰度比绝对偏差(ΔI_(绝对))为±20%,相对离子丰度比相对偏差(ΔI_(相对))为±50%。结论 本研究明确了GC-MS/MS检测血液基质中6种常见毒品定性判定的参考范围,对现有仪器分析方法的定性判定指标进行了有效补充。

Objective To establish and evaluate a gas chromatography-tandem mass spectrometry(GC-MS/MS) method for the detection of six common drugs(methamphetamine,meperidine,caffeine,codeine,cocaine and ketamine) in blood,and to improve the determination basis of results.Methods The above six drugs were added into the blank blood,and GC-MS/MS was used for detection after ether extraction.The collection,quantification and confirmation were carried out under the mode of multi-reaction monitoring(MRM).The qualitative results of the above six drugs were evaluated based on the maximum allowable deviation of the retention time and relative ion abundance ratio in the qualitative results of GC-MS/MS.Results There was a good linear relationship between the six common drugs,among which ketamine and caffeine had the lowest detection limit(0.01 μg/mL),methamphetamine had the highest detection limit(0.5 μg/mL).The retention time(RT) and relative retention time(RRT) of the target substance were stable under the six supplemental levels,and the absolute deviation(ΔRT_(absolute)) of RT was within ± 0.025 min.The absolute deviation of RRT(ΔRRT_(absolute)) was within ± 0.004.The relative ion abundance ratio absolute deviation(ΔI_(absolute)) is ± 20 %,and the relative ion abundance ratio relative deviation(ΔI_(relative)) is ± 50 %.Conclusion This study clarified the reference range for qualitative determination of six common drugs in blood matrix detected by GC-MS/MS,and effectively supplemented the qualitative determination indicators of existing instrumental analysis methods.