不同海拔栽培区甜樱桃休眠阶段花芽内含物变化差异

Difference of Changes in Flower Bud Inclusion of Sweet Cherry During Dormancy at Different Altitudes

以“布鲁克斯”甜樱桃为试材,采用差异比较、相关性分析等方法,研究了杭州高山和平地地区甜樱桃在休眠前、休眠中、花芽萌动前花芽内营养物质和内源激素差异,以期分析该地区甜樱桃休眠芽内生理状态。结果表明:杭州不同海拔地区均能满足“布鲁克斯”的有效低温积累量的需求。从休眠前至休眠中,高山甜樱桃花芽内可溶性蛋白质含量下降了25.89%,而平地值增加了8.31%,高山甜樱桃花芽内游离氨基酸含量下降了7.15%,而平地值增加了15.51%。但不同海拔地区甜樱桃从休眠前至花芽萌动前其花芽内可溶性蛋白质、淀粉、游离氨基酸整体变化趋势相同,含量均下降。总糖含量整体变化趋势及休眠中各组分含量变化趋势均相同,在不同的阶段,高山地区甜樱桃花芽内总糖含量均高于平地。内源激素含量变化在不同海拔地区存在明显差异。相关性分析结果表明,不同海拔地区甜樱桃在休眠过程中花芽内营养物质与内源激素的相关性不同。从休眠前至花芽萌动前,不同海拔地区甜樱桃花芽内大部分营养物质含量整体变化趋势相同,内源激素含量整体变化和阶段变化趋势在不同海拔地区间存在明显差异。

The sweet cherry‘Brooks’was used as the test material,methods of difference comparison and correlation analysis were adopted,the content of nutrients and endogenous hormone in flower buds was compared at different altitudes in Hangzhou,in order to analyze the physiological state of sweet cherry dormant bud in this area.The results showed that different altitudes could meet the demand of effective low temperature accumulation of‘Brooks’.From before dormancy to during dormancy,the soluble protein content in flower buds in high altitude region decreased by 25.89%,while the value in low altitude region increased by 8.31%.The free amino acid content in flower buds in high altitude region decreased by 7.15%,while the value in low altitude region increased by 15.51%.Whereas,the changes of soluble protein contents,starch contents and free amino acid contents in flower buds of sweet cherry at different altitudes regions were the same,they all decreased from before dormancy to before budbreak.The overall variation trend of total sugar content and the variation trend of each sugar component content in dormancy were the same.At different stages,the total sugar content in flower buds of sweet cherry in high altitude region was higher than that in low altitude region.The variation of endogenous hormone content was obviously different at different altitudes regions.The correlation analysis showed that the correlation between nutrients and endogenous hormones in flower buds was different at different altitudes.From before dormancy to before budbreak,the overall change trend of most nutrients in sweet cherry flower bud was the same at different altitudes,while the overall change trend and stage change trend of endogenous hormone content were significantly different at different altitudes.