姜黄挥发油的舒张血管活性及机制研究

Vasorelaxant activity and mechanism of essential oil from Curcuma longa L.

姜黄挥发油采用水蒸气蒸馏法从姜黄中提取得到。本文探讨了姜黄挥发油的舒张血管活性及机制。首先采用离体大鼠胸主动脉环实验发现姜黄挥发油具有舒张血管活性;通过去除血管环内皮和预孵育内皮型一氧化氮合酶(eNOS)抑制剂,发现姜黄挥发油的舒张血管活性具有内皮依赖性,且与eNOS有关;采用人脐静脉内皮细胞(HUVECs)模型,发现姜黄挥发油能够促进HUVECs中一氧化氮(NO)的生成,进一步说明了姜黄油的舒张血管作用与eNOS有关;最后免疫印迹实验结果表明姜黄挥发油能够上调磷脂酰肌醇3-激酶(PI3K)、蛋白激酶B(Akt)、eNOS的磷酸化水平。综上所述,姜黄挥发油具有舒张血管活性,且其机制与激活内皮PI3K/Akt/eNOS通路有关。本文中所有动物实验均获得成都中医药大学实验动物伦理委员会批准(批准号:2020-04)。

The essential oil from Curcuma longa L.(CLEO)was extracted by steam distillation.In this study,vasorelaxant activity and mechanism of CLEO was explored.Firstly,the experimental results of isolated rat thoracic aorta ring showed that the CLEO had vasorelaxant activity.By removing the endothelium of aorta ring and pre-incubating inhibitor of endothelial nitric oxide synthase(eNOS),it was found that the vasorelaxant activity of CLEO was endothelium-dependent and related to eNOS.By human umbilical vein endothelial cells(HUVECs)model,it was found that the CLEO could promote the production of nitric oxide(NO)in HUVECs,further indicating that the vasorelaxant activity of CLEO was related to eNOS.The results of Western blot showed that CLEO could up-regulate the phosphorylation levels of phosphatidylinositol 3-kinase(PI3K),protein kinase B(Akt)and eNOS.In summary,the CLEO has vasorelaxant activity,and its mechanism is related to activating the endothelial PI3K/Akt/eNOS pathway.All animal experiments in this paper were approved by the Committee of Laboratory Animal Welfare Ethics of Chengdu University of Traditional Chinese Medicine(approval No.2020-04).