Development and Validation of an Impedimetric Sensor Modified with Molecularly Imprinted Polymer for Direct Determination of Bisphenol A in Bioanalytics

There is a strong correlation between bisphenol A(BPA)and the risks of endocrine disruption in humans.However,the mechanism behind this correlation is still unknown due to the challenge of accurately assessing BPA levels in biological matrices.Herein,we demonstrate a molecularly imprinted polymer(MIP)modified impedimetric sensor treated with bovine serum albumin(BSA)not only reduces the impact of protein interference but also maintains both specificity and sensitivity for the precise analysis of BPA.The platinum disc electrode was coated with MIP via electrochemical polymerization of o-phenylenediamine(o-PD)in the presence of BPA as a template,and free sites were then blocked with 1%BSA.Electrochemical impedance spectroscopy(EIS)was utilized for the detection of BPA.The linear range for detection was between 1.0 pg/L and 10.0 ng/L,with a detection limit(LOD)of 0.28 pg/L.The sensor presented a good selectivity over structurally similar steroid hormones and BPA metabolites.To validate the performance of the proposed sensor in the presence of possible interfering proteins,it was used to quantify BPA in real samples without any prior treatment.These findings indicate that such an impedimetric sensor can be used to measure BPA in complex matrices.