棒柄杯伞的化学成分及其抗肿瘤活性研究

Chemical constituents from Clitocybe clavipes and their antitumor activities

对棒柄杯伞进行化学成分研究,采用硅胶、ODS、Sephadex LH-20柱色谱和半制备液相等多种色谱学技术对棒柄杯伞乙醇提取物进行分离纯化,通过^(1)H-NMR、^(13)C-NMR以及质谱等多种波谱学方法共鉴定出6个化合物,分别为clavilactone L(1)、clavilactone A (2)、clavilactone B (3)、clavilactone E (4)、clavilactone H (5)和clavilactone I (6)。其中,化合物1是1个新的苯并十元碳环混源萜。通过MTT法测定了化合物1~6的抗肿瘤活性,结果表明化合物1~6对人胃癌细胞(MGC-803)、人非小细胞肺癌细胞(A549)、宫颈癌细胞(HeLa)具有不同程度的增殖抑制作用。其中化合物1对人胃癌细胞MGC-803具有较显著的抑制活性,IC_(50)为11.76μmol·L^(-1)。

This paper aimed to study the chemical constituents from Clitocybe clavipes.Silica gel,ODS,Sephadex LH-20,and semi-preparative HPLC were employed to separate the ethanol extract of C.clavipes.Six compounds were identified by 1H-NMR,13CNMR,and ESI-MS as clavilactone L(1),clavilactone A(2),clavilactone B(3),clavilactone E(4),clavilactone H(5),and clavilactone I(6).Among them,compound 1 was a new meroterpenoid with a 10-membered carbocycle connected to a hydroquinone.The antitumor activities of compounds 1-6 were determined by the methyl thiazolyl tetrazolium(MTT)assay.The results showed that compounds 1-6 exerted inhibitory effects on the proliferation of human gastric cancer cells(MGC-803),human non-small cell lung cancer cells(A549),and cervical cancer cells(HeLa).Compound 1 exhibited significant inhibitory activity against MGC-803 cells,with the half maximal inhibitory concentration(IC_(50))of 11.76μmol·L^(-1).