利用猕猴桃雌雄同株材料发掘性别决定相关基因

Identification of Sex Determination-Related Genes from the Monoecious Kiwifruit Flowers

为探究猕猴桃雌雄异株变异为雌雄同株的分子机制,本研究对满天红猕猴桃雌雄同株变异株的雌花与雄花进行了转录组测序、生物信息学分析及q RT-PCR验证。转录组差异表达基因鉴定结果表明,雌花和雄花间共有337个差异表达基因,雄花中有241个基因表达量上调,有96个基因表达量下调。差异表达基因的GO与KEGG富集结果表明,与雌花相比,雄花中上调的基因主要参与氨基糖和核苷酸糖的代谢以及次生代谢物合成等路径;下调的基因则主要富集在次生代谢物合成、类胡萝卜素合成等途径。通过功能注释分析出30个潜在的性别相关调控基因,其中5个在次生代谢产物合成路径中表达。选取7个差异表达基因进行q RT-PCR验证,其在雌花、雄花中的表达水平与转录组数据一致。本研究结果为猕猴桃雌雄同株材料的创制提供了一定的理论依据。

In order to explore the molecular mechanism of the variation of kiwifruit from dioecious to monoecious,transcriptome sequencing,bioinformatics analysis and qRT-PCR verification on female and male flowers of the monoecious mutant of Mantianhong kiwifruit were carried out.The results of transcriptome differential expression gene identification showed that there were 337 differentially expressed genes(DEGs)between female and male flowers,with 241 genes up-regulated and 96 genes down-regulated in male flowers.The GO and KEGG enrichment of DEGs showed that compared with female flowers,the up-regulated genes in male flowers were mainly involved in the metabolism of amino sugars and nucleotide sugars and the synthesis of secondary metabolites.The down-regulated genes were mainly enriched in secondary metabolite synthesis,carotenoid synthesis and other pathways.Through functional annotation analysis,30 potential gender-related regulatory genes were identified,five of which were expressed in the secondary metabolite synthesis pathway.Seven DEGs were selected for qRT-PCR,and their expression levels in male and female flowers were in accordant with those in transcriptome data.The results of this study provide a theoretical basis for the creation of kiwifruit monoecious materials.