番茄红素对限制性应激雄鼠生长性能和肠道抗氧化功能的影响

Effects of Lycopene on Growth Performance and Intestinal Antioxidant Function in Male Mice under Restraint Stress

为了探讨番茄红素(LYC)对限制性应激雄鼠生长性能和肠道抗氧化功能的影响,本试验将80只雄鼠随机分为4个组:对照(CON)组、限制性应激(RS)组、LYC[10 mg/(kg·bw)]+限制性应激(LRS)组、玉米油+限制性应激(CRS)组。LRS组和CRS组每天在限制性应激处理前2 h分别灌胃LYC和玉米油(0.5 mL/只),限制性应激时长为5 h/d,连续处理14 d。每日记录各组小鼠应激前后体重和采食量,试验结束后取十二指肠、空肠和回肠组织,ELISA试剂盒检测组织中超氧化物歧化酶(SOD)活力、谷胱甘肽过氧化物酶(GSH-PX)活力、总抗氧化能力(T-AOC)和丙二醛(MDA)含量;Western blot检测组织中磷酸化蛋白激酶B/蛋白激酶B(p-AKT/AKT)、核因子E2相关因子(Nrf2)和血红素氧合酶1(HO-1)蛋白表达量。结果显示,与RS组相比,LRS组雄鼠应激前后体重减少量显著降低(P<0.05),平均日采食量有增加趋势(P>0.05);十二指肠、空肠和回肠组织中SOD活力、GSH-PX活力和T-AOC显著升高(P<0.05),MDA含量显著降低(P<0.05);回肠p-AKT/AKT显著升高(P<0.05),十二指肠和空肠p-AKT/AKT有升高趋势(P>0.05);十二指肠和空肠中Nrf2蛋白相对表达量显著升高(P<0.05),回肠中Nrf2蛋白相对表达量有升高趋势(P>0.05);十二指肠、空肠和回肠组织中HO-1蛋白相对表达量均显著升高(P<0.05)。CRS组以上指标与RS组相比均无显著差异(P>0.05),表明玉米油仅作为溶剂并不发挥缓解作用。结果表明,番茄红素可缓解限制性应激对雄鼠生长性能的不利影响,有效提高雄鼠肠道局部抗氧化水平,其机制可能与激活AKT-Nrf2/HO-1通路有关。

To investigate the effects of lycopene(LYC) on growth performance and intestinal antioxidant function in male mice under restraint stress,80 male mice were randomly divided into four groups:Control(CON) group,Restraint stress(RS) group,LYC [10 mg/(kg·bw)]+restraint stress(LRS) group,and Corn oil+restraint stress(CRS) group.The LRS and CRS groups were gavaged with LYC and corn oil(0.5 mL/mouse) respectively,2 hours before restraint stress treatment.The duration of restraint stress was 5 hours per day,continuously for 14 days.Daily records of body weight and feed intake before and after stress were kept.At the end of the experiment,tissues from the duodenum,jejunum,and ileum were collected.ELISA kits were used to detect the activities of superoxide dismutase(SOD),glutathione peroxidase(GSH-PX),total antioxidant capacity(T-AOC),and the content of malondialdehyde(MDA) in the tissues.Western blot analysis was performed to detect the protein expression levels of phosphorylated protein kinase B/protein kinase B(p-AKT/AKT),nuclear factor erythroid 2-related factor(Nrf2),and heme oxygenase 1(HO-1).The results showed that compared with the RS group,the weight loss of male mice before and after stress in the LRS group was significantly reduced(P<0.05),and the average daily feed intake showed an increasing trend(P>0.05).The activities of SOD,GSH-PX,and T-AOC in the duodenum,jejunum,and ileum tissues were significantly increased(P<0.05),while the MDA content was significantly decreased(P<0.05).The p-AKT/AKT level in the ileum was significantly increased(P<0.05),with an increasing trend in the duodenum and jejunum(P>0.05).The relative expression levels of Nrf2 protein in the duodenum and jejunum were significantly increased(P<0.05),with an increasing trend in the ileum(P>0.05).The relative expression levels of HO-1 protein in the duodenum,jejunum,and ileum tissues were significantly increased(P<0.05).The CRS group showed no significant differences in the above indicators compared to the RS group(P>0.05),indicating that corn oil,serving only as a solvent,does not have a mitigating effect.These results indicate that lycopene can alleviate the adverse effects of restraint stress on the growth performance of male mice and effectively enhance the local antioxidant level in the intestines.The mechanism may be related to the activation of the AKT-Nrf2/HO-1 pathway.