急性低氧胁迫对马氏珠母贝生长、免疫及矿化相关基因表达的影响

Effects of Acute Hypoxic Stress on Expression of Growth-,Immunity-,and Biomineralization-related Genes of Pinctada fucata martensii

【目的】探究急性低氧胁迫对马氏珠母贝(Pinctada fucata martensii)生长、免疫及矿化相关基因表达的影响,为马氏珠母贝抗逆品系选育提供参考。【方法】将马氏珠母贝从常氧条件下移至2 mg·L^(-1)低溶解氧水体,比较其在低氧胁迫0、12、24和48 h时免疫与抗氧化相关基因(caspase-3、HSP90、CAT和SOD)、生长相关基因(EGFR、FGF18、OSR1和tgfbr1)和矿化相关基因(pif177、aspein、nacrein和TIMP)的表达水平。【结果】急性低氧胁迫后,HSP90的表达水平12 h时低于对照组(P <0.05),caspase-3和CAT的表达在胁迫24 h时显著增加(P <0.05),而SOD的表达无明显变化(P> 0.05);矿化相关基因pif177、aspein、nacrein在急性低氧胁迫后的表达均显著降低,TIMP仅在12 h时显著降低(P <0.05);生长相关基因EGFR、FGF18和OSR1基因的表达也出现显著下降(P <0.05),tgfbr1的表达在24 h时显著高于对照组(P <0.05)。【结论】急性低氧胁迫上调免疫与抗氧化相关基因中caspase-3、CAT的表达,抑制HSP90的表达,抑制矿化相关基因的表达,抑制生长相关基因中EGFR、FGF18和OSR1的表达,上调tgfbr1的表达。

【Objective】 To investigate the effects of acute hypoxic stress on the expression of growth-,immunity-and of biomineralization-related genes of Pinctada fucata martensii,and to provide a reference for the selection and breeding of stress-resistant strains of P.fucata martensii.【Method】The expression levels of immune and antioxidant activity-related genes(caspase-3,HSP90,CAT,and SOD),growthrelated genes(EGFR,FGF18,OSR1,and tgfbr1),as well as biomineralization-related genes(pif177,aspein,nacrein,and TIMP) of P.fucata martensii at time points of 0,12,24,and 48 hours under hypoxic stress were compared,when the pearl oyster was moved from normoxic conditions to a water body with low dissolved oxygen at 2 mg·L^(-1).【Result】 After acute hypoxic stress,the expression level of HSP90was lower than that of the control group at 12 h(P < 0.05),and the expression levels of caspase-3 and CAT were significantly increased at 24 h of stress(P < 0.05),whereas there was no significant change in the expression of SOD(P > 0.05);The expression of biomineralization-related genes pif177,aspein,and nacrein were all significantly reduced after acute hypoxic stress,and TIMP was significantly reduced only at 12 h(P < 0.05).The expression of growth-related genes EGFR,FGF18,and OSR1 genes also showed a significant decrease(P < 0.05),and the expression of tgfbr1 was significantly higher than that of the control group at 24 h(P < 0.05).【Conclusion】 Acute hypoxic stress up-regulated the expression of caspase-3 and CAT but suppressed the expression of HSP90 of immune and antioxidant-related genes,suppressed the expression of biomineralization-related genes,suppressed the expression of EGFR,FGF18,and OSR1 but up-regulated the expression of tgfbr1 of growth-related genes.