摘要
Pathogen identification for infectious disease diagnosis is crucial and can be achieved through direct isolation of microbes or indirect detection based on the host immune system. Clinical metagenomic next-generation sequencing(mNGS) is an emerging technology that comprehensively analyzes genetic material(DNA and RNA) from both microbes and the host. It does not rely on prior knowledge of a pathogen's genome. This article reviews the clinical utility of mNGS in diagnosing infectious diseases and current technical difficulties.
作者
陈宏斌
詹明华
刘思
Francois Balloux
王辉
Hongbin Chen;Minghua Zhan;Si Liu;Francois Balloux;Hui Wang(Department of Clinical Laboratory,Peking University People’s Hospital,Beijing 100044,China;UCL Genetics Institute,University College London,London WC1E 6BT,UK)
基金
supported by the National Key Research and Development Program of China (2022YFA1304300,2018YFE0102100)
the National Natural Science Foundation of China (81625014)
Beijing Municipal Science and Technology Commission Program (Z191100006619100)。
