摘要
Dear Editor,CRISPR/Cas systems,which can create novel mutations by manipulating the genome or transcriptome,have been developed as a powerful molecular tool for gene editing in various organisms(Cheng et al.,2022;Gao,2021).However,identifying mutations in large samples and decoding chimeric mutations are time-consuming,labor-intensive.
基金
supported by the National Natural Science Foundation of China(32025028,U20A2030)
Zhejiang Provincial Key Research and Development Program(2021C02063-6)
Earmarked Fund(CARS--01)
the Hainan Seed Industry Laboratory(B23CJ0208)
Zhejiang Provincial Natural Science Foundation of China(LTGN24C130005)。