CDC6和MCM6的表达与宫颈癌的相关性

Study on the correlation between the express of CDC6 and MCM6 and cervical cancer

目的探讨宫颈癌组织中细胞分裂周期蛋白6(CDC6)和微小染色体维持蛋白6(MCM6)蛋白及mRNA的表达水平与宫颈癌临床病理特征的关系。方法收集2018年10月—2020年11月就诊于华北理工大学附属医院妇科的94例患者的宫颈组织标本,其中44例经病理学确诊的宫颈癌组织作为宫颈癌组,50例因子宫肌瘤行全子宫切除术患者的正常宫颈组织作为对照组。采用免疫组织化学法(IHC)检测两组宫颈组织中CDC6、MCM6蛋白的表达情况;采用实时荧光定量PCR法(qRT-PCR)检测两组宫颈组织中CDC6、MCM6 mRNA的表达量。结果宫颈癌组中CDC6、MCM6蛋白的阳性表达率明显高于对照组(P<0.01)。在宫颈癌组织中CDC6、MCM6蛋白的阳性表达率与宫颈癌患者的淋巴结转移、临床分期和HPV16/18型感染相关(P<0.05),与是否绝经、肿块大小、宫旁浸润、分化程度、组织类型无关(P>0.05)。CDC6、MCM6 mRNA在宫颈癌组中的表达量高于对照组(P<0.05);当CDC6或MCM6 mRNA的表达量增加时,发生宫颈癌的风险增加(OR:2.591,95%CI:1.126~5.962;OR:4.239,95%CI:1.780~10.095)。在MCM6蛋白阳性表达的宫颈癌组织中,CDC6蛋白的阳性表达率更高(P<0.05)。结论CDC6、MCM6蛋白和mRNA的表达与宫颈癌的发生发展有一定的关系,两者可作为宫颈癌的生物学指标。

Objective To investigate the expression levels o cell division cyclin(CDC6)and microchromosome maintenance protein(MCM6)protein and mRNA in cervical pain tissues and their relationship with the clinicopathologic features of cervical cancer.Methods From October 2018 to November 2020,the patients admitted to the Department of Gynecology of the Affiliated Hospital of North China University of Science and lechnology were collected as subjects.The expressions of CDC protein and MCM6 protein in 44 cases of cervical cancer and 50 cases of normal cervical tissues were detected by immunohistochemical method.Real-time quantitative fluorescence PCR(qRT-PCR)was used to detect the expressions of CDC mRNA and MCM6 mRNA in the cervical tissues of the above two groups.Results The positive expression rate of CDC6 protein and MCM6 protein in cervical cancer group was significantly higher than that in normal cervical cancer group,the difference was statistically significant(P<0.01).The positive expression rates of CDC6 protein and MCM6 protein in cervical cancer tissues were correlated with lymph node metastasis,clinical stage and HPV16/18 infection of cervical cancer patients,and the differences were statistically significant(P<0.05),but not related to menopause,mass size,parastatal infiltration,degree of differentiation and tissue type(P>0.05).The expression level of MCM6 mRNA in cervical cancer group was higher than that in normal cervical cancer group,and the difference between the two groups was statistically significant(P<0.05).When the exoression of CDC6 mRNA or MCM6 mRNA was increased,the risk of cervical cancer was increased(OR:2.591,95%CI:1.126~5.962;OR:4.239,95%CI:1.780~10.095).In cervical cancer tissues with positive expression of MCM6 protein,the positive expression rate of CDC protein was higher,and the difference was statistically significant(P<0.05).Conclusion The expressions of CDC6 and MCM6 protein and mRNA are related to the occurrence and development of cervical cancer,which can be used as biological indicators of cervical cancer.