两株高效解磷菌的筛选及解磷性能分析

Screening and Analysis of Two Strains of High Efficiency Phosphor-Soluble Bacteria

为获得能够把难溶性磷转化为可溶性磷的解磷微生物,从茅台酒厂种植地的土壤中分离筛选出两株具有解磷能力的菌株,并对这两株菌株进行分子生物学鉴定及解磷性能分析,测定其发酵液中的有机酸,初步探究解磷机理和在酒糟中的生长能力。结果表明,筛选的P1和P2菌株分别为路氏肠杆菌(Enterobacter ludwigii)和分散泛菌(Pantoea dispersa)。P1和P2单菌培养的难溶磷降解率分别在第10天和第1天达到最高9.62%和7.56%,P1和P2混菌(P3)培养难溶磷的降解率在第2天达到最大为18.83%。不同条件下菌株对难溶磷的降解率有一定影响,其中40℃混菌培养在第6天高达33.82%。P1主要产乙酸,高达4103.65 mg/L,P2主要产乙酸和琥珀酸,混菌培养主要产乙酸,其次是草酸。两株菌在酒糟中具有较强的生长能力,为解磷功能菌株制备酒糟生物有机肥提供一定的参考。

In order to obtain phosphorus soluble microorganisms that can convert insoluble phosphorus into soluble phosphorus,two strains with phosphorus soluble ability were isolated and screened from soil of planting field of Maotai Distillery,and molecular biological identification and phosphorus soluble performance of both strains analysis were carried out,and organic acids in their fermentation broth were determined,and the phosphorus soluble mechanism and growth ability in distillers′grains were initially explored.The results showed that the selected strain P1 and strain P2 were Enterobacter ludwigii and Panthenia dispersus respectively.Regarding the degradation rate of insoluble phosphorus,the highest was at 9.62% and 7.56% in P1 and P2 single culture on the 10 d and 1 d respectively,and 18.83% in P1 and P2 mixed culture on the 2 d.Under different conditions,the degradation rate of insoluble phosphorus was affected to some extent,and the mixed culture at 40℃ reached as high as 33.82% on 6 d.P1 mainly produced acetic acid,up to 4103.65 mg/L,P2 mainly produced acetic acid and succinic acid,mixed culture mainly produced acetic acid,followed by oxalic acid.The two strains had strong growth ability in distillers′grains,which provided a certain reference for preparation of bioorganic fertilizer with distillers′grains.