食管鳞状细胞癌患者癌组织和癌旁正常组织中RELT mRNA含量与其预后的关系

Relationship between RELT mRNA levels and prognosis in cancer tissues and adjacent normal tissues of patients with esophageal squamous cell carcinoma

目的探究食管癌鳞状细胞癌(ESCC)患者癌组织与癌旁正常组织中表达于淋巴样组织的受体(RELT)mRNA含量与其预后的关系。方法收集2019年1月至2021年1月在信阳市中心医院行手术切除的100例ESCC组织标本作为观察组,选择对应癌旁的正常组织(距癌组织5 cm以上)作为对照组,比较两组组织RELT mRNA相对表达量的差异。依据RELT mRNA相对表达量中位数将ESCC患者分为低表达(45例)和高表达(55例)两组,比较两组患者临床资料,对ESCC患者随访24个月,绘制Kaplan-Meier曲线比较两组患者的生存时间,COX回归模型分析患者预后的相关危险因素。结果观察组RELT mRNA相对表达量及RELT蛋白相对表达量分别为8.03±2.31、0.87±0.08,均高于对照组(3.05±0.71、0.63±0.07)(P<0.05)。以RELT mRNA相对表达量中位数8.05作为分界值,将100例ESCC患者分为高表达组(≥8.05)和低表达组(<8.05),高表达组ESCC患者年龄<60岁、肿瘤浸润深度T3+T4、TNM分期ⅡB~Ⅲ期、淋巴结转移及脉管侵犯发生率均高于低表达组ESCC患者(P<0.05)。随访24个月,高表达组ESCC患者生存时间为(15.85±1.02)个月,低表达组ESCC患者生存时间为(19.71±0.84)个月,高表达组ESCC患者生存时间短于低表达组(Log-rank检验χ^(2)=6.371,P=0.012)。经COX回归模型分析,TNM分期ⅡB~Ⅲ期(OR=1.377,95%CI:1.032~1.837)、RELT mRNA相对表达量≥8.05(OR=1.420,95%CI:1.057~1.910)为ESCC预后不良的危险因素(P<0.05)。结论RELT mRNA在ESCC癌组织中表达升高,与患者的不良预后有关,可作为临床ESCC预后预测以及治疗的潜在靶点。

【Objective】To investigate the relationship between receptor expressed in lymphoid tissue(RELT)mRNA content and prognosis in cancer tissues and adjacent normal tissues of patients with esophageal squamous cell carcinoma(ESCC).【Methods】A hundred ESCC tissue samples surgically removed in Xinyang Central Hospital from January 2019 to January 2021 were collected as the observation group,and the corresponding normal tissues adjacent to cancer(larger than 5 cm away from cancer tissues)were selected as the control group,and the relative expression levels of RELT mRNA in the two groups were compared.According to the median RELT mRNA expression,ESCC patients were divided into 2 groups with low expression(45 cases)and high expression(55 cases).The difference of clinical data between the two groups was compared.The patients with ESCC were followed up for 24 months,and the survival time of the two groups was compared by drawing Kaplan-Meier curve.Multivariate COX proportional hazard regression model was used to analyze the risk factors of poor prognosis in ESCC patients.【Results】The relative expression levels of RELT mRNA and RELT protein in ESCC patients in observation group were 8.03±2.31 and 0.87±0.08,which were higher than those in control group(3.05±0.71 and 0.63±0.07)(P<0.05).Using the median RELT mRNA expression of 8.05 as the cut-off value,100 ESCC patients were divided into high expression group(≥8.05)and low expression group(<8.05).The incidence of age<60 years old,tumor invasion depth T3+T4,TNM stageⅡB-Ⅲ,lymph node metastasis and vascular invasion in ESCC patients in high expression group were higher than those in low expression group(P<0.05).After 24 months of follow-up,the survival time of ESCC patients in the high RELT mRNA expression group was 15.85±1.02 months,and the survival time of ESCC patients in the low RELT mRNA expression group was 19.71±0.84 months.The survival time of ESCC patients in the high RELT mRNA expression group was shorter than that in the low RELT mRNA expression group(log-rank testχ^(2)=6.371,P=0.012<0.05).TNM stagesⅡB toⅢ(OR=1.377,95%CI:1.032-1.837)and RELT mRNA relative expression≥8.05(O^R=1.420,95%CI:1.057-1.910)were risk factors for poor prognosis of ESCC(P<0.05).【Conclusion】The increased expression of RELT mRNA in ESCC cancer tissues is associated with poor prognosis of patients,and can be used as a potential target for prognosis prediction and treatment of ESCC.