大豆GmZAT10基因克隆与表达分析

Cloning and expression analysis of GmZAT10 gene in Soybean

非生物胁迫对植物的生长和发育具有严重的影响.研究发现植物中存在NAC、bZIP、WRKY、AP2/ERF、MYC、bHLH、C2H2等一系列可以调控非生物胁迫的转录因子.为了揭示GmZAT10基因的功能,从大豆中克隆出GmZAT10基因,并对该基因进行载体构建、生物信息学分析、亚细胞定位和实时荧光定量PCR(qRT-PCR)等研究.结果表明,GmZAT10全长702bp,共编码233个氨基酸,分子量为59.14kDa,等电点为4.85;亚细胞定位结果显示GmZAT10定位于细胞核和细胞膜中;通过同源蛋白序列对比显示,GmZAT10蛋白具有两个C2H2锌指结构域,含有植物所特有的QALGGH和C2H2保守序列.通过qRT-PCR技术检测大豆不同组织和不同处理条件下GmZAT10基因的表达情况可知,该基因在大豆根中表达量较高,并且受到高低温、NaCl和ABA诱导表达.结果表明GmZAT10在大豆非生物胁迫中发挥作用.

Abiotic stress has a very serious impact on plant growth and development.Studies have found that there are a series of transcription factors in plants that can regulate abiotic stress,such as NAC,bZIP,WRKY,AP2/ERF,MYC,bHLH and C2H2.In order to reveal the function of GmZAT10 gene,gene cloning,vector construction,bioinformatics analysis,subcellular localization and real-time quantitative PCR(qRT-PCR)were carried out.The results showed that GmZAT10 was 702 bp in length and encoded 233 amino acids,The molecular weight is 59.14 kDa and the isoelectric point is 4.85.Subcellular localization showed that GmZAT10 was localized in the nucleus and cell membrane.GmZAT10 has two C2H2 zinc finger domains and contains QALGGH and C2H2 conserved sequences that are unique to plants.qRT-PCR was used to detect the expression of GmZAT10 gene in different soybean tissues and under different treatment conditions.The results showed that GmZAT10 gene was highly expressed in soybean roots and was induced by high and low temperature,NaCl and ABA.This study suggests that GmZAT10 play an important role in abiotic stress in soybean.