11种加工食品中多种核苷/核苷酸同步定量分析的超高效液相色谱-质谱法方法建立

Simultaneous Analysis of Multiple Nucleosides/Nucleotides in 11 Kinds of Processed Food by Ultra-High-Performance Liquid Chromatography-Tandem Mass Spectrometry

核苷酸、核苷广泛分布于各类食品及调味品中。文章建立了一种适用于加工食品的同步检测11种核苷/核苷酸类物质(腺苷、鸟苷、胞苷、尿苷、肌苷、胸苷、腺苷酸、鸟苷酸、胞苷酸、尿苷酸、肌苷酸)的超高效液相色谱-质谱法。结果表明,该方法下被分析化合物所属浓度范围内线性关系良好,5种核苷酸的线性范围是0.080~80.000 μg/mL,6种核苷的线性范围分别在0.002~2.000 μg/mL和0.005~5.000 μg/mL,相关系数均在0.9920以上,各组分均得到很好分离。检出限为0.293~9.186 ng/mL,定量限为2.000~80.000 ng/mL,精密度、重复性、稳定性良好;干样品加标平均回收率为91.9%~106.3%,湿样品加标平均回收率为86.5%~97.2%。采用该方法对市售11种食品中核苷和核苷酸含量进行了定量分析,结果与已报道的相近,说明方法具备可行性和可靠性。与传统的高效液相色谱相比,文章建立的超高效液相色谱-质谱法具有前处理简单、分析时间短、成本更低等优点,为加工食品中核苷/核苷酸类物质的同时检测提供了基础研究支持,为相关产品的开发和质量检测提供技术支持。

Nucleotides and nucleosides are widely distributed in various types of food and condiments.A method using ultra-high-performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS) was developed for the simultaneous detection of 11 nucleotide/nucleoside (adenosine,guanosine,cytidine,uridine,inosine,thymidine,adenosine monophosphate,guanosine monophosphate,cytidine monophosphate,uridine monophosphate,and inosine monophosphate) in processed foods.The results showed that the developed method had good linearity within the concentration range of the analyzed compounds.The linear range for the five nucleosides was from 0.080 μg/mL to 80.000 μg/mL,while for the six nucleotides,it was 0.002–2.000 μg/mL and 0.005–5.000 μg/mL,respectively,with the correlation coefficients above 0.9920,and each component was well separated.The limits of detection ranged from 0.293 to 9.186 ng/mL,and the limits of quantification ranged from 2.000 ng/mL to 80.000 ng/mL.The method exhibited good precision,repeatability,and stability,with average recovery rates ranging from 91.9% to 106.3% for dry sample,and average recovery rates ranging from 86.5% to 97.2% for wet sample.The method was applied to the quantitative analysis of nucleotides and nucleosides content in 11 commercially available food samples,and the results were in agreement with previously reported values,demonstrating the feasibility and reliability of the method.This developed UPLC-MS/MS method offers advantages over traditional high-performance liquid chromatography,including simple sample preparation,shorter analysis time,and lower cost.It provides a basic for simultaneous detection of nucleotide/nucleoside substances in processed food and serves as technical support for the development and quality testing of related products.