聚乙二醇对盐碱胁迫下花楸胚胎萌发及幼苗生理指标的影响

Effects of Polyethylene Glycol on Embryo Germination and Physiological Indicators of Sorbus pohuashanensis Empryos under Saline Alkali Stress

采用室内培养皿发芽法,研究外源添加聚乙二醇、碳酸钠、碳酸氢钠对花楸胚胎萌发及幼苗生理指标的影响。结果表明,聚乙二醇处理花楸胚胎后使萌发率提高15%~25%,碳酸钠、碳酸氢钠处理使花楸胚胎的萌发率分别降低了88%和85%。聚乙二醇可缓解盐碱胁迫对花楸胚胎萌发的抑制,使胁迫下胚胎的萌发率提高1~2倍。聚乙二醇可提高盐碱胁迫下花楸胚胎中抗氧化酶活性,使胁迫下胚胎中超氧化物歧化酶(SOD)、过氧化物酶(POD)和过氧化氢酶活性(CAT)分别提高16.45%、75%、80%。聚乙二醇使胁迫下胚胎中的丙二醛(MDA)含量降低41.44%。研究显示,聚乙二醇能够有效缓解盐碱胁迫对花楸胚胎的抑制作用,并提高花楸胚胎中的抗氧化酶活性,增强花楸的抗逆性,为建立PEG引发促进花楸胚胎萌发技术提供科学依据。

This experiment used an indoor culture dish germination method to study the effects of exogenous addition of polyethylene glycol,sodium carbonate,and sodium bicarbonate on the embryos germination and physiological indicators of S.pohuashanensis seedlings.The results showed that polyethylene glycol treatment increased the germination percentage of S.pohuashanensis embryos by 15%to 25%,while sodium carbonate and sodium bicarbonate treatment reduced the germination percentage of S.pohuashanensis embryos by 88%and 85%,respectively.Polyethylene glycol can alleviate the inhibition of saline alkali stress on the germination of S.pohuashanen⁃sis embryos,and increase the germination rate of embryos under stress by 1-2 times.Polyethylene glycol can increase the activity of antioxidant enzymes in the embryos of S.pohuashanensis under saline alkali stress,resulting in an increase of 16.45%,75%,and 80%in enzyme activities such as SOD,POD,and CAT,respectively.Polyethylene glycol reduced MDA in embryos under stress by 41.44%.Research has shown that polyethylene glycol can effectively alleviate the inhibitory effect of salt alkali stress on the embryos of S.pohuasha⁃nensis,improve the activity of antioxidant enzymes in S.pohuashanensis embryos,enhance the stress resistance of S.pohuashanensis,and provide scientific basis for establishing PEG priming technology to promote the germination of S.pohuashanensis embryos.