高效液相色谱法检测玉米中呕吐毒素方法优化

Optimization of high performance liquid chromatography for detection of vomitoxin in maize

在GB 5009.111—2016《食品安全国家标准食品中脱氧雪腐镰刀菌烯醇及其乙酰化衍生物的测定》基础上,采用高效液相色谱法,通过优化样品的前处理过程,旨在建立一种能够快速、准确、大批量检验玉米中呕吐毒素含量的测定方法。结果显示,呕吐毒素在100~5000 ng/mL的浓度范围内具有较好的线性关系,线性相关系数R^(2)大于0.99997,方法检出限为18.4μg/mL,定量限为61.3μg/mL,样品回收率在90.04%~108.42%,相对标准偏差(RSD)为3.69%~6.70%,符合国标对呕吐毒素的检验要求,满足大规模玉米样品的快速测定。

On the basis of GB 5009.111—2016"Determination of deoxynivalenol and its acetylated derivatives in foodstuffs",high-performance liquid chromatography(HPLC)was used,and by optimizing the pretreatment process of the samples,the aim was to establish an assay method that could rapidly,accurately,and in large quantities test the content of vomitoxin in corn.The results demonstrated that the linear relationship of vomitoxin was good in the concentration range of 100~5000 ng/mL,with the linear correlation coefficient(R^(2))greater than 0.99997.The limit of detection(LOD)of the method was 18.4μg/mL,the limit of quantification(LOQ)was 61.3μg/mL,the recoveries of vomitoxin were in the range of 90.04%~108.42%,and the relative standard deviations(RSD)were in the range of 3.69%~6.70%,which complied with the requirements of the national standard for the determination of vomitoxin in corn.It met the requirements for the rapid determination of large-scale corn samples.