冠心病患者ABO、Rh、Kidd、Kell血型特点调查分析

Investigation and analysis of ABO,Rh,Kidd,Kell blood group characteristics in patients with coronary heart disease

目的探讨ABO、Rh、Kidd、Kell血型系统抗原在冠心病患者中的分布特点。方法收集新疆维吾尔自治区人民医院就诊符合纳入排除标准的冠心病患者样本2000例,进行ABO、Rh、Kidd、Kell血型主要抗原鉴定,并对RhD血型阴性表型的基因外显子测序,对不同民族冠心病患者血型分布情况进行统计分析。结果冠心病患者ABO血型中O型占比为28.87%;维吾尔族为A>B>O>AB,哈萨克族趋势同维吾尔族。检出RhD阴性样本59例(3.32%),频率依次为维吾尔族>哈萨克族>柯尔克孜族>回族>其他民族>蒙古族;检测出13种Rh分型,频率最高的是柯尔克孜族的CcDEe,为38.81%,各民族表型分布符合Hardy-Weinberg平衡(P>0.05);而Rh血型基因型分布,e基因频率仅次于D基因,而基因组合体频率中CDe基因型最常见;D阴性样本基因测序发现了新的突变位点5号外显子g.712G>A,另发现1例RhD 1227A杂合型Del样本。Jk^(a)、Jk^(b)抗原分布哈萨克族最高达83.33%、78.57%,对Jk^(a)/Jk^(b)表型统计发现哈萨克族Jk(a+b+)频率达61.90%,未检出Jk(a-b-)表型。K抗原检测得到阳性样本30例,占比1.69%,柯尔克孜族阳性率最高,为2.24%。结论ABO、Rh、Kidd、Kell血型系统抗原在不同民族冠心病患者中分布有各自的特点,为冠心病患者输血前检测、临床输血治疗及后期疾病相关性研究提供了依据。

Objective To investigate the distribution characteristics of ABO,Rh,Kidd and Kell blood group system antigens in patients with coronary heart disease(CHD).Methods Samples of 2000 patients with CHD who met the inclusion and exclusion criteria were collected from the People's Hospital of Xinjiang Uygur Autonomous Region.Major antigens of ABO,Rh,Kidd and Kell blood groups were identified,gene exons of RhD blood group negative phenotype were sequenced,and blood group distribution of CHD patients of different ethnic groups was statistically analyzed.Results The proportion of ABO type O in CHD patients was 28.87%.Uyghur was A>B>O>AB,and Kazakh tended to be the same as Uyghur.RhD negative samples were detected in 59 cases(3.32%),and the frequency was Uyghur>Kazak>Kirgiz>Hui>Other nationalities>Mongolian.Thirteen Rh types were detected,the CcDEe of Kirgiz was 38.81%,and the phenotypic distribution of all ethnic groups was in line with Hardy-Weinberg equilibrium(P>0.05).In the distribution of Rh genotype,the frequency of e gene was second only to D gene,while CDe genotype was the most common in the frequency of gene combinations.The gene sequencing of D-negative samples found a new mutation site,exon 5 g.712G>A,and a heterozygous Del sample of RhD 1227A.The distribution of Jk^(a)and Jkb antigens in Kazakh population was up to 83.33%and 78.57%,and the frequency of Jk(a+b+)in Kazakh population was found to be 61.90%,and no Jk(a-b-)phenotype was detected.The positive K antigen was detected in 30 cases,accounting for 1.69%,the highest positive rate of Kirgiz was 2.24%.Conclusion The distribution of ABO,Rh,Kidd and Kell blood group system antigens in patients with CHD of different nationalities has its own characteristics,which provides a basis for the detection of CHD before transfusion,clinical transfusion treatment and later disease correlation research.